Cytek Biosciences Cytek® Aurora

Full-spectrum cytometer enabling high-parameter cellular analysis with precision.

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Features

  • 3 to 5 laser options for flexible panel design
  • Up to 64 fluorescence detectors
  • Full-spectrum detection with unmixing algorithms
  • User-friendly SpectroFlo® software
  • High-resolution multiparameter analysis

Applications

  • Immunophenotyping
  • Cancer and stem cell research
  • Clinical biomarker discovery
  • Functional immunology
  • Single-cell analysis
Cytek Biosciences Cytek® Aurora

Product Specifications

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PERFORMANCE

Detection Sensitivity
FITC 222 5 MESF2 PE 222 4 MESF2 APC 222 3 MESF2 Pacific Blue 222 4 MESF
Dynamic Range
224bit4 645 log decades
Event Rate
Up to 359000 events9sec
Sample Throughput
1555560 55L5min 5manual55 100 55L5min 5plate55 965well in 27 min 5ASL5
Resolution
High resolution8 down to 70 nm with ESP888
Coincidence Rate
Not specified
Flow Stability
Robust vacuum fluidics2 flat2top beam profile

WAVELENGTH AND DETECTION

Number of Detectors
Up to 67 664 fluorescence + 3 scatter6
Fluorescence Channels
64 channels 1405 nm1 161 488 nm1 141 640 nm1 81 561 nm1 101 355 nm1 161
Light Scatter Parameters
FSC8 Blue SSC8 Violet SSC 8down to 70888100 nm resolution8
Detector Type
CWDM semiconductor arrays 1fluorescence11 high1performance SSC1FSC detectors
Filter Sets
No filter changes needed
Emission Wavelength Range
365888829 nm
Excitation Wavelength(s)
405 nm4 488 nm4 640 nm 4base44 355 nm4 561 nm 4upgrades4
Compensation Capabilities
Spectral unmixing with wizard 7SpectroFlo777
Optical Design
Fixed optics9 5 spatial lasers9 fused silica cuvette

OPTICS AND LIGHT SOURCE

Number of Lasers
Up to 5 lasers 3upgradeable from 33
Laser Wavelengths
355 nm2 405 nm2 488 nm2 561 nm2 640 nm
Laser Power
355 nm6 20 mW6 405 nm6 100 mW6 488 nm6 50 mW6 561 nm6 50 mW6 640 nm6 80 mW
Laser Configuration
Upgradeable from 3 to 5 lasers
Beam Shaping Technology
Flat9top beam9 narrow vertical height
Laser Alignment
Auto0adjusted during QC

SAMPLE HANDLING

Sample Input Format
12x75 mm tubes1 9613841well plates1 deep1well plates1 401tube racks
Sample Volume Requirements
Not specified
Flow Cell Type
Fused silica cuvette
Sample Flow Rate Control
Low6Med6High6manual 6amp6 ASL optimized rates
Fluidics System
Vacuum4based4 multiple cleaning modes
Sheath Fluid Requirements
20 L cubitainers
Sample Loading
Manual or Automated 4ASL4AMS4
Clogging Detection & Prevention
Not specified4 includes purge4clean modes
Cleaning & Debubbling Mechanisms
SIT flush6 purge filter6 clean flow cell6 long clean

DATA ACQUISITION AND ANALYSIS

Data Acquisition Software
SpectroFlo22
Parameters Per Event
Area4 Height 4all44 Width 4scatter + 1 fluoro4laser4
Compensation Tools
Spectral unmixing with controls
Real-Time Data Analysis
Yes0 real0time unmixing
Histogram/Scatter Plot Tools
Yes6 standard plots with SpectroFlo66
Gating Strategies
Gating supported with flexible thresholds
Multi Parameter Analysis
Up to 40 colors0 deep immunoprofiling
Data Export Formats
FCS 361

AUTOMATION AND SOFTWARE

Automated Startup & Shutdown
Yes5 standard fluidics modes
Remote Operation Capabilities
Not specified
Scheduling & Batch Processing
Supported via ASL5AMS
API / LIMS Integration
Not specified
Advanced Analysis Software
SpectroFlo111 OMIQ1 FlowSOM1 FCS Express
Quality Control Features
Daily QC2 automated module2 QC beads
Compliance Tools
Not specified

OPERATIONAL FEATURES

User Interface
Intuitive6 workflow6based
Ease of Use
Easy setup1 minimal training required
Maintenance Requirements
Service plans4 2 maintenance visits4year optional
Durability & Reliability
Long5term lab use implied5 service contracts available
Operating Conditions
155552855C5 20555855 RH5 100555250 VAC5 dust5free
Noise & Vibration Levels
Not specified
Power Supply Requirements
100888140 VAC8 15 A or 200888250 VAC8 10 A
Dimensions and Weight
Not specified
Cooling System
500 W dissipation5 specific system not described

FEATURES AND APPLICATIONS

Specialized Assays
PrimeFlow444 RNA4 yeast4tumor AF extraction4 small particle detection
Sorting Capability
Not standard8 improved resolution with CS software
Cell Sorting Modes
Not specified
Rare Event Detection
Yes5 high resolution supports rare population detection
High-Throughput Screening Compatibility
Yes8 via ASL8AMS
Clinical vs. Research Use
For Research Use Only
Multiplexing Capability
Up to 407color panels7 high flexibility
Customizable Panels
Yes3 compatible with many fluorochromes

GENERAL INFORMATION

Product Type
Spectral Flow Cytometry System 8Cytek Aurora8888
Documentation and Support
SpectroLearn0000 Webinars0 Blog0 Docs0 Phone Support
Upgrade Options
33to35 lasers3 ESP333 Detection Option
Regulatory Certifications
Class 1 Laser Product
Connectivity
Cytek11 Cloud1 ASL integrated1 network1API not specified
Compatibility
Fluorochromes excited by onboard lasers7 common lab formats
Warranty & Service Agreements
Value4 Premium4 Preventive4 includes parts4 labor4 support

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