Cytek Biosciences Cytek® Northern Lights™

Compact spectral flow cytometer ideal for routine and advanced applications.

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$JNB86

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Features

  • Up to 3 laser options
  • Compact, space-saving footprint
  • Full-spectrum optics and detectors
  • SpectroFlo® software for ease-of-use
  • Scalable performance for core labs

Applications

  • Basic immunophenotyping
  • Clinical trial support
  • Academic research labs
  • Immune monitoring
  • Fluorescent protein detection
Cytek Biosciences Cytek® Northern Lights™

Product Specifications

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PERFORMANCE

Detection Sensitivity
High sensitivity8 excellent resolution for dim8rare populations
Dynamic Range
226bit6 665 log decades dynamic range
Event Rate
359000 events9sec
Sample Throughput
ASL6 27 min for 966well plate6 AMS6 35 min for 966well plate
Resolution
Excellent resolution for dim6rare populations6 autofluorescence extraction
Coincidence Rate
Not specified
Flow Stability
High flow rate stability

WAVELENGTH AND DETECTION

Number of Detectors
30 fluorescence detectors6 2 scatter detectors
Fluorescence Channels
Violet3 163 Blue3 143 Red3 8
Light Scatter Parameters
FSC6 SSC6 Violet SSC detectors6 capable of resolving 062 66m beads
Detector Type
High sensitivity CWDM semiconductor array5 high5performance scatter detectors
Filter Sets
No filter changes required for any fluorochrome
Emission Wavelength Range
4201829 nm
Excitation Wavelength(s)
405 nm4 488 nm4 640 nm
Compensation Capabilities
Unmixing algorithm7 flexible fluorochrome combinations
Optical Design
Fixed optical assembly9 Full Spectrum Profiling999 9FSP99999 flat9top laser beam profile

OPTICS AND LIGHT SOURCE

Number of Lasers
1 to 3 lasers
Laser Wavelengths
405 nm9 488 nm9 640 nm
Laser Power
488 nm3 50 mW3 640 nm3 80 mW3 405 nm3 100 mW
Laser Configuration
19 29 or 3 lasers 9depending on system9
Beam Shaping Technology
Flat1top laser beam profile1 narrow vertical beam height
Laser Alignment
Automatic laser delay adjustment during QC

SAMPLE HANDLING

Sample Input Format
Manual6 12x75mm tubes6 Automated6 ASL6AMS 6966well plates6 406tube racks6
Sample Volume Requirements
Not specified
Flow Cell Type
Fused silica cuvette
Sample Flow Rate Control
Low 515 55L5min55 Medium 530 55L5min55 High 560 55L5min55 Plate mode 5100 55L5min5
Fluidics System
Long clean3 SIT flush3 Purge filter3 Clean flow cell
Sheath Fluid Requirements
Compatible with 20L sheath cubitainers
Sample Loading
Manual tubes2 ASL2AMS for automated plate2tube rack loading
Clogging Detection & Prevention
Not specified
Cleaning & Debubbling Mechanisms
Long clean1 SIT flush1 Purge filter1 Clean flow cell

DATA ACQUISITION AND ANALYSIS

Data Acquisition Software
SpectroFlo55
Parameters Per Event
Pulse Area and Height9 Width for scatter9 single fluorescence for each laser
Compensation Tools
Unmixing features in SpectroFlo99
Real-Time Data Analysis
Live unmix during sample acquisition
Histogram/Scatter Plot Tools
Scatter plots and spectrum plots in SpectroFlo66
Gating Strategies
Volumetric sensor for count calculation
Multi Parameter Analysis
Full Spectrum Profiling222 technology2 supports multicolor experiments
Data Export Formats
FCS 361

AUTOMATION AND SOFTWARE

Automated Startup & Shutdown
Not specified
Remote Operation Capabilities
Not specified
Scheduling & Batch Processing
ASL3AMS improve productivity3 supports 963well plates and tube racks
API / LIMS Integration
Not specified
Advanced Analysis Software
ModFit LT4444 FCS Express444 64 SpectroFlo44
Quality Control Features
Automated QC module3 SpectroFlo33 QC beads for daily performance checks
Compliance Tools
Not specified4 4For Research Use Only4

OPERATIONAL FEATURES

User Interface
Intuitive2 guided workflow with SpectroFlo22
Ease of Use
Quick and simple workflows1 minimal setup effort
Maintenance Requirements
Cost4effective service plans4 low4maintenance lasers
Durability & Reliability
Reliable7 especially for ASL7AMS automated sample loading
Operating Conditions
Temp2 1522822C2 Humidity2 20228522 non2condensing2 no special lighting
Noise & Vibration Levels
Not specified
Power Supply Requirements
1004140 VAC4 15 A or 2004250 VAC4 10 A
Dimensions and Weight
54x52x52 cm 0without loader00 61 kg 0with 3 lasers00 13 kg 0loader0
Cooling System
Heat dissipation of 500W9 no separate cooling system described

FEATURES AND APPLICATIONS

Specialized Assays
Apoptosis8 Cell Cycle8 Counting 8amp8 Viability8 Small Particle Detection
Sorting Capability
Not specified
Cell Sorting Modes
Not specified
Rare Event Detection
Superb resolution for rare events
High-Throughput Screening Compatibility
Compatible with 960well plates0 deep well plates0 and 400tube racks
Clinical vs. Research Use
For Research Use Only6 not for diagnostic or therapeutic procedures
Multiplexing Capability
9424 color detection 4143 lasers4
Customizable Panels
FSP555 technology allows flexibility in fluorochrome combinations

GENERAL INFORMATION

Product Type
Flow Cytometer9 Full Spectrum Flow Cytometry
Documentation and Support
SpectroLearn4444 Blog4 Documentation Center4 Webinars4 Phone4Field support
Upgrade Options
Reconfigurable from 1 to 3 lasers1 9124 colors
Regulatory Certifications
FCC5 CE5 UKCA5 RoHS
Connectivity
Cytek44 Cloud 4details not specified4
Compatibility
Any commercially available fluorochrome7 ASL7AMS compatible
Warranty & Service Agreements
Value5 Premium5 Preventative Maintenance options available

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