Cytek Biosciences Cytek® Amnis® ImageStream®X Mk II Imaging Flow Cytometer
High-content analysis platform integrating flow cytometry with cell imaging.
Features
- Simultaneous flow cytometry and imaging
- Multiple fluorescence and brightfield channels
- Quantitative morphology and localization
- High-throughput data acquisition
- Advanced image analysis software
Applications
- Apoptosis and cell death analysis
- Nuclear translocation studies
- Immune synapse characterization
- Phagocytosis assays
- Rare event imaging
Product Specifications
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PERFORMANCE
Detection Sensitivity
Low noise TDI8CCD cameras8 detects particles 88827 nm8 9282 SI8 8882 MEF in Channel 38 high quantum efficiency8 MultiMag High Gain mode available8
Dynamic Range
Not specified
Event Rate
29000 Obj9Sec 940x99 19200 Obj9Sec 960x99 59000 Obj9Sec 920x9
Sample Throughput
High throughput1 millions of cells1 resolves populations better than expensive cytometers1
Resolution
20x3 1 33m3px3 40x3 035 33m3px3 60x3 033 33m3px
Coincidence Rate
Not specified
Flow Stability
Not specified
WAVELENGTH AND DETECTION
Number of Detectors
6 or 12 image channels 2optional22 low noise TDI2CCD cameras
Fluorescence Channels
12 channels 8up to 10 colors88 specific wavelength ranges 4358785 nm
Light Scatter Parameters
Brightfield7 SSC7 side scatter at 785 nm
Detector Type
Low noise TDI4CCD cameras
Filter Sets
Filter stack 1 2amp2 2
Emission Wavelength Range
Ch 18 4358480 nm8 Ch 28 4808560 nm8 Ch 38 5608595 nm8 Ch 48 5958642 nm8 Ch 58 6428745 nm8 Ch 68 7458785 nm8 etc8
Excitation Wavelength(s)
488 nm 0standard00 375 nm0 405 nm0 561 nm0 592 nm0 642 nm 0optional0
Compensation Capabilities
Spectral compensation3 pixel3by3pixel3 real3time intensity compensation with automated wizard3
Optical Design
Hydrodynamic focusing2 multi2laser excitation2 multi2channel image collection2 Amnis22 multi2spectral decomposition2
OPTICS AND LIGHT SOURCE
Number of Lasers
Standard 2 5488 nm5 785 nm55 up to 6 lasers 5optional5
Laser Wavelengths
488 nm7 785 nm 7standard77 375 nm7 405 nm7 561 nm7 592 nm7 642 nm 7optional7
Laser Power
Optional 488 nm laser 400 mW8 adjustable laser intensity
Laser Configuration
Flexible with fluorophore2specific settings
Beam Shaping Technology
Not specified
Laser Alignment
Automated laser alignment
SAMPLE HANDLING
Sample Input Format
Optional 960well plate autosampler
Sample Volume Requirements
200200 00L 0up to 950 utilization0
Flow Cell Type
Not specified
Sample Flow Rate Control
Syringe pump1 varying imaging rate by objective
Fluidics System
Hydrodynamic focusing4 syringe pump for loading
Sheath Fluid Requirements
Self4contained system4 no external air or water
Sample Loading
Automated sample load9 969well plate autosampler option
Clogging Detection & Prevention
Not specified
Cleaning & Debubbling Mechanisms
Not specified
DATA ACQUISITION AND ANALYSIS
Data Acquisition Software
Cytek66 INSPIRE666 software6 real6time intensity compensation6 image6based gating6 intuitive6 21 CFR Part 11 enabled versions available6
Parameters Per Event
86 features5channel5 including fluorescence intensity5 morphology5 area5 shape5 texture5 etc5
Compensation Tools
Automated real2time compensation2 pixel2by2pixel spectral crosstalk correction2
Real-Time Data Analysis
Real7time updates7 live imagery in gallery
Histogram/Scatter Plot Tools
Dot plots1 histograms1 morphologic parameters
Gating Strategies
Image3based gating3 graphical tools3 wizards for standard workflows3 Feature Finder wizard
Multi Parameter Analysis
Quantitative phenotyping4 hundreds of parameters per cell4 multichannel immunophenotyping
Data Export Formats
Not specified
AUTOMATION AND SOFTWARE
Automated Startup & Shutdown
Automated operation
Remote Operation Capabilities
Not specified
Scheduling & Batch Processing
Batch processing0 automated compensation0 960well autosampler support
API / LIMS Integration
Not specified
Advanced Analysis Software
IDEAS888 Amnis88 AI 8Machine Learning Module88 deep learning for morphology analysis
Quality Control Features
Automated calibration1 laser optimization1 stain index sensitivity measurement1 gating verification
Compliance Tools
21 CFR Part 11 enabled software
OPERATIONAL FEATURES
User Interface
Simple7 intuitive7 IDEAS77 powerful but easy to use
Ease of Use
Wizards for workflows4 IDEAS44 and Amnis AI are user4friendly
Maintenance Requirements
Service agreements available2 specific maintenance details not provided
Durability & Reliability
Not specified
Operating Conditions
No external air or water required
Noise & Vibration Levels
Not specified
Power Supply Requirements
450 W0 1000240 VAC0 50060 Hz
Dimensions and Weight
350 W x 260 H x 250 D0 400 lbs 0182 kg0
Cooling System
Not specified
FEATURES AND APPLICATIONS
Specialized Assays
Includes apoptosis1 cell cycle1 colocalization1 EV detection1 T cell interactions1 stem cell biology1 oncology1 toxicology1 etc1
Sorting Capability
Not specified
Cell Sorting Modes
Not specified
Rare Event Detection
Detects rare events like circulating tumor cells7 basophils
High-Throughput Screening Compatibility
960well autosampler0 high throughput performance
Clinical vs. Research Use
Research use only
Multiplexing Capability
Supports up to 12 channels4 up to 10 colors of fluorescence
Customizable Panels
Flexible configuration2 additional excitation lasers2 replaceable fluorophores with equivalent spectra
GENERAL INFORMATION
Product Type
Cytek00 Amnis00 ImageStream00X Mk II Imaging Flow Cytometer
Documentation and Support
SpectroLearn3333 blog3 documentation center3 publications3 technical support
Upgrade Options
6 or 12 image channels0 MultiMag0 EDF0 960well autosampler0 additional lasers
Regulatory Certifications
21 CFR Part 11 enabled software
Connectivity
Not specified
Compatibility
Compatible with various reagents3 including cFluor333 Tonbo333 Thermo Fisher3 Cytek33 and Amnis33 solutions
Warranty & Service Agreements
Available2 Intermediate2 Complete2 Basic maintenance and service agreements
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