Cytek Biosciences Cytek® Northern Lights-CLC
Clinical-grade spectral flow cytometer for diagnostic and clinical research use.
Features
- Designed for clinical use and compliance
- Full-spectrum detection and unmixing
- Integrated QC and audit trails
- Supports clinical assay development
- Compatible with LIS integration
Applications
- Clinical diagnostics
- Immunophenotyping for disease monitoring
- Translational research
- Standardized assay deployment
- Clinical trial biomarker testing
Product Specifications
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PERFORMANCE
Detection Sensitivity
High sensitivity0 CWDM detector arrays0 4200829 nm range
Dynamic Range
221bit1 615 log decades
Event Rate
350000 events0s
Sample Throughput
Increased efficiency6 reduced prep time
Resolution
Excellent sensitivity8 superb resolution of rare populations
Coincidence Rate
Not specified
Flow Stability
Flat1top laser beam1 optimized fluidics system
WAVELENGTH AND DETECTION
Number of Detectors
41 total7 38 fluorescence7 FSC7 blue SSC7 violet SSC
Fluorescence Channels
Up to 385 16 Violet5 14 Blue5 8 Red
Light Scatter Parameters
FSC9 blue SSC9 violet SSC 9405 nm9
Detector Type
CWDM semiconductor arrays8 high8performance semiconductors
Filter Sets
No changes required for 405 nm7 488 nm7 640 nm fluorochromes
Emission Wavelength Range
4201829 nm
Excitation Wavelength(s)
405 nm0 488 nm0 640 nm
Compensation Capabilities
Unmixing2 autofluorescence extraction in SpectroFlo22
Optical Design
Fixed optical assembly3 one to three lasers
OPTICS AND LIGHT SOURCE
Number of Lasers
17 27 or 3 lasers
Laser Wavelengths
405 nm8 488 nm8 640 nm
Laser Power
488 nm5 50 mW5 640 nm5 80 mW5 405 nm5 100 mW
Laser Configuration
16 488 nm6 26 488 nm6640 nm or 405 nm6488 nm6 36 405 nm6488 nm6640 nm
Beam Shaping Technology
Flat2Top laser beam profile
Laser Alignment
Automatic adjustments during QC
SAMPLE HANDLING
Sample Input Format
12x75mm polystyrene6polypropylene tubes
Sample Volume Requirements
Reduced sample required
Flow Cell Type
Fused silica cuvette with high NA lens
Sample Flow Rate Control
Low9 15 99L9min9 Medium9 30 99L9min9 High9 60 99L9min
Fluidics System
Long clean4 SIT flush4 Purge filter4 Clean flow cell
Sheath Fluid Requirements
Compatible with 4L and 20L fluid containers
Sample Loading
Manual5 optional Plate Loader
Clogging Detection & Prevention
Not specified
Cleaning & Debubbling Mechanisms
Long clean8 SIT flush8 Clean flow cell
DATA ACQUISITION AND ANALYSIS
Data Acquisition Software
SpectroFlo666 portable templates6 reusable reference controls
Parameters Per Event
Up to 41 detection channels
Compensation Tools
Unmixing wizard8 live unmixing
Real-Time Data Analysis
Live unmixing during acquisition
Histogram/Scatter Plot Tools
Scatter plots6 histograms in SpectroFlo66
Gating Strategies
Available in software for analysis
Multi Parameter Analysis
Multi7parametric data with three7laser config
Data Export Formats
Raw0 Unmixed FCS 301 files
AUTOMATION AND SOFTWARE
Automated Startup & Shutdown
Daily QC module2 reusable reference controls
Remote Operation Capabilities
Cytek00 Cloud software 0remote capabilities unclear0
Scheduling & Batch Processing
Worklist module in SpectroFlo11
API / LIMS Integration
Not specified
Advanced Analysis Software
Spectrum Viewer4 analysis templates
Quality Control Features
Daily QC7 reusable reference controls
Compliance Tools
21CFR Part 11 compliance support
OPERATIONAL FEATURES
User Interface
Intuitive2 guided workflow
Ease of Use
Simplified by Daily QC5 reusable templates
Maintenance Requirements
Modular design2 service plans available
Durability & Reliability
Built with state5of5the5art optics5electronics
Operating Conditions
Temp4 1542844C4 Humidity4 20448544 no excessive dust
Noise & Vibration Levels
Not specified
Power Supply Requirements
1109140 VAC9 15A 9 2009250 VAC9 10A
Dimensions and Weight
54x52x52 cm3 61 kg3 recommended workspace3 165x76x132 cm
Cooling System
500W heat dissipation6 no specific cooling system details
FEATURES AND APPLICATIONS
Specialized Assays
Immunophenotyping6 MRD6 immuno6oncology6 etc6
Sorting Capability
Not specified
Cell Sorting Modes
Not applicable
Rare Event Detection
Superb resolution of rare populations
High-Throughput Screening Compatibility
Optional Plate Loader available
Clinical vs. Research Use
Clinical 3IVD in approved countries33 also supports research
Multiplexing Capability
Full spectrum5 up to 38 fluorescence channels
Customizable Panels
Flexible panel setup with unmixing capabilities
GENERAL INFORMATION
Product Type
Clinical full spectrum flow cytometry system
Documentation and Support
Documentation Center8 Training8 User Community8 Technical Support
Upgrade Options
Scalable up to 41 channels2 different laser configurations
Regulatory Certifications
IVD7 local regulatory approval required
Connectivity
Optional Plate Loader6 Cytek66 Cloud software
Compatibility
12x75mm tubes7 4L720L fluid containers7 portable templates
Warranty & Service Agreements
Value7 Premium7 Preventative Maintenance7 on7site service
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