Revvity EnVision XCite Multimode Plate Reader

Reading Accuracy

Abs @ 2 OD: <2%; Precision @ 2 OD: <0.1%

Absorbance Range

0–4 OD (200 µL & 50 µL); 0–3 OD (7.5 µL)

Sensitivity

Fluor (Filter): <4 pM; Mono: <10 pM; TRF: <55 fM; TRF Laser: <15 fM; Alpha: <100 amol; Luminescence: <5 amol (US)

Measurement Speed

Kinetic: 6–20 s; Single read: 22–36 s; Full plate times vary by assay and format (5 s–28 min)

Dynamic Range

Same as Absorbance Range

Wavelength Range

Absorbance: 230–1000 nm; Fluorescence: 230–850 nm

Wavelength Selection

Tunable in 0.1 nm increments (both absorbance and fluorescence)

Measurement Technology

Multimode: Absorbance, Fluorescence, TRF, Alpha, Luminescence, Fluorescence Polarization (filter/mono/laser options)

Detector

High-speed detector

Optical System

Hybrid (Filter + Monochromator); Modular mirror design

Light Source

Xenon flash lamp

Excitation and Emission Filters

User-changeable, barcode ID; includes full filter list and recommendations

Autofocus

Not specified

Plate Shaking

Linear, Orbital, Dual Orbital

Microplate Formats

1–3456 wells

Reagent Injector System

Optional 2-channel; 2–475 µL, 1 µL steps; pump speed 100–500 µL/s

Plate Reader Mode

Multimode

Data Acquisition

Times per mode/plate format provided; no detailed process

Data Analysis

Signals VitroVivo software

Automation

Compatible with multiple systems; robotic integration supported

Software

Signals VitroVivo; Enhanced Security option (21 CFR Part 11)

Ease of Use

User-changeable optics; barcode ID; automation compatible

Maintenance and Durability

Minimal downtime; global support

Operating Conditions

2 °C above ambient to 55 °C; <10 min to 37 °C

Power and Cooling

100–240V, 50/60 Hz; Cooling: Not specified

Dimensions and Footprint

H: 580 mm; D: 550 mm; W: 420 mm; Weight: 50 kg

Brand and Model

EnVision™ XCite multimode plate reader

Documentation and Support

Global network of >300 support staff; filter/dye support

Compatibility and Connectivity

Automation systems; barcode reader; CAN card interface

Configuration and Options

Fully upgradeable; Optional: Alpha, Mono, Laser TRF, US Luminescence, Quad mono, injectors, stackers, Enhanced Security

Licensing and Certification

21 CFR Part 11 support; SelectScience® Seal of Quality; For research use only

Cost and Operational Expenses

Not specified

Thermo Fisher Scientific Varioskan™ LUX Multimode Microplate Reader Catalog no-VLBL00GD2

Reading Accuracy

±0.003 Abs or ±2% (200–399 nm), ±0.003 Abs or ±1% (400–1000 nm); Precision <0.001 Abs; Linearity ±2%

Absorbance Range

0–6 Abs

Sensitivity

Fluorescein <0.4 fmol/well; ATP <7 amol/well

Measurement Speed

15 s (96-well), 45 s (384-well), 135 s (1536-well)

Dynamic Range

Fluorescence >6 decades; Luminescence >7 decades; Auto-range selection

Wavelength Range

Abs: 200–1000 nm; Fluorescence Exc: 200–822 nm, Em: 270–840 nm; Lum: 360–670 nm

Wavelength Selection

Double monochromator (Abs, Fluorescence); Filters (TRF)

Measurement Technology

Abs, FI (incl. FRET), Lum, AlphaScreen/AlphaLISA, TRF (incl. TR-FRET/hTRF)

Detector

Photomultiplier Tube (PMT)

Optical System

Filter + monochromator; Double monochromator (Abs, FI), filters (TRF)

Light Source

Xenon Flash Lamp

Excitation and Emission Filters

Filters (TRF); Emission filters purchasable

Autofocus

Not specified

Plate Shaking

Orbital

Microplate Formats

6–1536 well; Abs: 6–384 well; μDrop Plates supported

Reagent Injector System

0–2 dispensers; 2–5000 μl, 1 μl steps; Simultaneous dispense & measure

Plate Reader Mode

Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra, Spectral Scanning

Data Acquisition

Real-time spectra/curves; Continuous data save

Data Analysis

Built-in calc.; Export: *.xlsx, *.pdf, *.xml, *.txt; Email support

Automation

Compatible; Auto range, safety controls, self-diagnostics

Software

SkanIt PC Software (Research & Drug Discovery Editions); Multilingual; Free

Ease of Use

Auto range, safety controls, virtual pipetting, user-friendly UI

Maintenance and Durability

Auto calibration and self-diagnostics

Operating Conditions

Temp: +4° to 45°C; CO₂: 0.1–15%; O₂: 1–21%; Integrated gas control

Power and Cooling

Not specified

Dimensions and Footprint

58×53×51 cm (23×21×20 in); 57 kg (125 lb)

Brand and Model

Thermo Scientific™ Varioskan™ LUX

Documentation and Support

Contact sales; Docs and certificates available

Compatibility and Connectivity

PC software interface (SkanIt)

Configuration and Options

Modular; Top/Bottom read; 0–2 dispensers; Filters available

Licensing and Certification

HTRF™, DLReady™, 21 CFR Part 11 (Drug Disc. Ed.)

Cost and Operational Expenses

Not specified