Thermo Fisher Scientific Varioskan™ LUX Multimode Microplate Reader Catalog no-VLBL00GD1

Reading Accuracy

0.003 Abs or ±2% (200–399 nm), ±1% (400–1000 nm); SD <0.001 Abs, CV <0.5% @ 450 nm

Absorbance Range

0–6 Abs; Linearity: 0–4 Abs (96-well), 0–3 Abs (384-well) @ 450 nm

Sensitivity

< 0.4 fmol fluorescein/well; < 7 amol ATP/well

Measurement Speed

15s (96-well), 45s (384-well), 135s (1536-well); < 2.2s/well spectral scan

Dynamic Range

Fluorescence > 6 decades; Luminescence > 7 decades; auto range selection

Wavelength Range

Abs: 200–1000 nm; Fluor Ex: 200–822 nm, Em: 270–840 nm; Lumi: 360–670 nm

Wavelength Selection

Double monochromators; filters for TRF

Measurement Technology

Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen, TRF

Detector

Photomultiplier Tube (PMT)

Optical System

Filter and monochromator optics

Light Source

Xenon Flash Lamp

Excitation and Emission Filters

Emission filters available; filters used in TRF

Autofocus

Not specified

Plate Shaking

Orbital

Microplate Formats

6–1536-well plates; Absorbance: up to 384-well; μDrop compatible

Reagent Injector System

0, 1, or 2 dispensers; 2–5000 μL in 1 μL steps; supports simultaneous dispense

Plate Reader Mode

Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra

Data Acquisition

Continuous save; real-time spectra/kinetics display

Data Analysis

Built-in calculations; export: *.xlsx, *.pdf, *.xml, *.txt

Automation

Auto range selection; auto calibration; self-diagnostics

Software

SkanIt Software; PC interface; license free; includes virtual pipetting tool

Ease of Use

Intuitive UI; safety controls; simplified setup

Maintenance and Durability

Auto calibration; self-diagnostics; designed for reliability

Operating Conditions

Temp: Ambient +4° to 45°C; CO₂: 0.1–15%; O₂: 1–21%

Power and Cooling

Not specified

Dimensions and Footprint

58 × 53 × 51 cm; 57 kg

Brand and Model

Thermo Scientific Varioskan LUX

Documentation and Support

Docs & certificates available; contact support

Compatibility and Connectivity

PC software interface; Connect: Lab, Data, Apps

Configuration and Options

Modular; top/bottom read; 0–2 dispensers; filters sold separately

Licensing and Certification

HTRF Certified; DLReady validated; 21 CFR Part 11 (Drug Discovery Edition)

Cost and Operational Expenses

Not specified

Revvity EnVision XCite Multimode Plate Reader

Reading Accuracy

Abs @ 2 OD: <2%; Precision @ 2 OD: <0.1%

Absorbance Range

0–4 OD (200 µL & 50 µL); 0–3 OD (7.5 µL)

Sensitivity

Fluor (Filter): <4 pM; Mono: <10 pM; TRF: <55 fM; TRF Laser: <15 fM; Alpha: <100 amol; Luminescence: <5 amol (US)

Measurement Speed

Kinetic: 6–20 s; Single read: 22–36 s; Full plate times vary by assay and format (5 s–28 min)

Dynamic Range

Same as Absorbance Range

Wavelength Range

Absorbance: 230–1000 nm; Fluorescence: 230–850 nm

Wavelength Selection

Tunable in 0.1 nm increments (both absorbance and fluorescence)

Measurement Technology

Multimode: Absorbance, Fluorescence, TRF, Alpha, Luminescence, Fluorescence Polarization (filter/mono/laser options)

Detector

High-speed detector

Optical System

Hybrid (Filter + Monochromator); Modular mirror design

Light Source

Xenon flash lamp

Excitation and Emission Filters

User-changeable, barcode ID; includes full filter list and recommendations

Autofocus

Not specified

Plate Shaking

Linear, Orbital, Dual Orbital

Microplate Formats

1–3456 wells

Reagent Injector System

Optional 2-channel; 2–475 µL, 1 µL steps; pump speed 100–500 µL/s

Plate Reader Mode

Multimode

Data Acquisition

Times per mode/plate format provided; no detailed process

Data Analysis

Signals VitroVivo software

Automation

Compatible with multiple systems; robotic integration supported

Software

Signals VitroVivo; Enhanced Security option (21 CFR Part 11)

Ease of Use

User-changeable optics; barcode ID; automation compatible

Maintenance and Durability

Minimal downtime; global support

Operating Conditions

2 °C above ambient to 55 °C; <10 min to 37 °C

Power and Cooling

100–240V, 50/60 Hz; Cooling: Not specified

Dimensions and Footprint

H: 580 mm; D: 550 mm; W: 420 mm; Weight: 50 kg

Brand and Model

EnVision™ XCite multimode plate reader

Documentation and Support

Global network of >300 support staff; filter/dye support

Compatibility and Connectivity

Automation systems; barcode reader; CAN card interface

Configuration and Options

Fully upgradeable; Optional: Alpha, Mono, Laser TRF, US Luminescence, Quad mono, injectors, stackers, Enhanced Security

Licensing and Certification

21 CFR Part 11 support; SelectScience® Seal of Quality; For research use only

Cost and Operational Expenses

Not specified

Thermo Fisher Scientific Varioskan™ LUX Multimode Microplate Reader Catalog no-VLBL00GD2

Reading Accuracy

±0.003 Abs or ±2% (200–399 nm), ±0.003 Abs or ±1% (400–1000 nm); Precision <0.001 Abs; Linearity ±2%

Absorbance Range

0–6 Abs

Sensitivity

Fluorescein <0.4 fmol/well; ATP <7 amol/well

Measurement Speed

15 s (96-well), 45 s (384-well), 135 s (1536-well)

Dynamic Range

Fluorescence >6 decades; Luminescence >7 decades; Auto-range selection

Wavelength Range

Abs: 200–1000 nm; Fluorescence Exc: 200–822 nm, Em: 270–840 nm; Lum: 360–670 nm

Wavelength Selection

Double monochromator (Abs, Fluorescence); Filters (TRF)

Measurement Technology

Abs, FI (incl. FRET), Lum, AlphaScreen/AlphaLISA, TRF (incl. TR-FRET/hTRF)

Detector

Photomultiplier Tube (PMT)

Optical System

Filter + monochromator; Double monochromator (Abs, FI), filters (TRF)

Light Source

Xenon Flash Lamp

Excitation and Emission Filters

Filters (TRF); Emission filters purchasable

Autofocus

Not specified

Plate Shaking

Orbital

Microplate Formats

6–1536 well; Abs: 6–384 well; μDrop Plates supported

Reagent Injector System

0–2 dispensers; 2–5000 μl, 1 μl steps; Simultaneous dispense & measure

Plate Reader Mode

Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra, Spectral Scanning

Data Acquisition

Real-time spectra/curves; Continuous data save

Data Analysis

Built-in calc.; Export: *.xlsx, *.pdf, *.xml, *.txt; Email support

Automation

Compatible; Auto range, safety controls, self-diagnostics

Software

SkanIt PC Software (Research & Drug Discovery Editions); Multilingual; Free

Ease of Use

Auto range, safety controls, virtual pipetting, user-friendly UI

Maintenance and Durability

Auto calibration and self-diagnostics

Operating Conditions

Temp: +4° to 45°C; CO₂: 0.1–15%; O₂: 1–21%; Integrated gas control

Power and Cooling

Not specified

Dimensions and Footprint

58×53×51 cm (23×21×20 in); 57 kg (125 lb)

Brand and Model

Thermo Scientific™ Varioskan™ LUX

Documentation and Support

Contact sales; Docs and certificates available

Compatibility and Connectivity

PC software interface (SkanIt)

Configuration and Options

Modular; Top/Bottom read; 0–2 dispensers; Filters available

Licensing and Certification

HTRF™, DLReady™, 21 CFR Part 11 (Drug Disc. Ed.)

Cost and Operational Expenses

Not specified