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$SRC35PERFORMANCE
Reading Accuracy
Absorbance Range
Sensitivity
Measurement Speed
Dynamic Range
MASS RANGE AND ANALYTE DETECTION
Wavelength Range
Wavelength Selection
Measurement Technology
IONIZATION, SOURCE AND INTERFACE
Detector
Optical System
Light Source
Excitation and Emission Filters
MASS ANALYZER
Autofocus
Plate Shaking
CHROMATOGRAPHY AND SAMPLE HANDLING
Microplate Formats
Reagent Injector System
Plate Reader Mode
DATA ACQUISITION AND ANALYSIS
Data Acquisition
Data Analysis
AUTOMATION AND SOFTWARE
Automation
Software
OPERATIONAL FEATURES
Ease of Use
Maintenance and Durability
Operating Conditions
Power and Cooling
Dimensions and Footprint
GENERAL INFORMATION
Brand and Model
Documentation and Support
Compatibility and Connectivity
Configuration and Options
Licensing and Certification
Cost and Operational Expenses
Thermo Fisher Scientific Varioskan™ LUX Multimode Microplate Reader Catalog no-VLB000D0
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$IVBM1PERFORMANCE
Reading Accuracy
±0.003 Abs or ±2% (200–399 nm); ±0.003 Abs or ±1% (400–1000 nm); SD < 0.001 Abs; CV < 0.5% @ 450 nm
Absorbance Range
0–6 Abs
Sensitivity
<0.4 fmol (top), <4 fmol (bottom), fluorescein/well
Measurement Speed
15 s (96-well), 45 s (384-well), 135 s (1536-well), <2.2 s/well scan
Dynamic Range
>6 decades (top), >5.5 decades (bottom)
MASS RANGE AND ANALYTE DETECTION
Wavelength Range
Abs: 200–1000 nm; Exc: 200–822 nm; Em: 270–840 nm
Wavelength Selection
Double monochromators (Abs & Fluorescence)
Measurement Technology
Absorbance, Fluorescence, Luminescence, AlphaScreen/LISA, TRF
IONIZATION, SOURCE AND INTERFACE
Detector
Photomultiplier Tube (PMT)
Optical System
Filter + monochromator optics
Light Source
Xenon Flash Lamp
Excitation and Emission Filters
Monochromators + optional filters available
MASS ANALYZER
Autofocus
Not specified
Plate Shaking
Orbital
CHROMATOGRAPHY AND SAMPLE HANDLING
Microplate Formats
6–1536 wells, μDrop; Abs: up to 384-well
Reagent Injector System
Optional: 0, 1, or 2 dispensers; supports simultaneous dispensing
Plate Reader Mode
Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra
DATA ACQUISITION AND ANALYSIS
Data Acquisition
Continuous data saving
Data Analysis
Built-in calculations, real-time display, one-click export, QC tools
AUTOMATION AND SOFTWARE
Automation
Auto range selection, calibration, self-diagnostics; automation-compatible
Software
SkanIt (PC), Research & Drug Discovery Editions, virtual pipetting
OPERATIONAL FEATURES
Ease of Use
Auto setup, smart safety controls, intuitive software
Maintenance and Durability
Auto calibration, self-diagnostics, high reliability
Operating Conditions
Ambient +4°C to 45°C, optional gas control module
Power and Cooling
100/240 V, 50/60 Hz; cooling not specified
Dimensions and Footprint
58×53×51 cm (23×21×20 in), 54 kg (119 lb)
GENERAL INFORMATION
Brand and Model
Thermo Scientific Varioskan LUX
Documentation and Support
Available; contact sales for more info
Compatibility and Connectivity
PC Software, Automation-compatible, HTRF & DLReady certified
Configuration and Options
Modular; various tech, reading modes, dispensers; optional gas module
Licensing and Certification
SkanIt free license; Drug Discovery: 21 CFR Part 11, HTRF, DLReady
Cost and Operational Expenses
Not specified
Revvity EnVision Nexus Multimode Microplate Reader
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$REBFMPERFORMANCE
Reading Accuracy
Abs: <0.05% at 0.5 OD; Precision: <0.01% at 0.5 OD
Absorbance Range
0–4 OD (50 µL)
Sensitivity
FI: <2 pM; FP: <1.15 mP SD; TRF: <0.02 pM; TRF Laser: <0.004 pM; Alpha HTS: 0.2 ng/mL; Alpha Enhanced: 0.4 ng/mL; Lum: <0.25 pM; Enhanced Lum: <0.025 pM; US Lum: <0.001 pM
Measurement Speed
384-well FI: 13 sec (typical); Max: 14 sec; US-LUM: 00:40
Dynamic Range
Not specified
MASS RANGE AND ANALYTE DETECTION
Wavelength Range
Not specified
Wavelength Selection
Filter-based; assay-ready preconfigured filters
Measurement Technology
Abs, FI, FP, TRF, TRF Laser, Lum, US-LUM, Alpha HTS/Enhanced
IONIZATION, SOURCE AND INTERFACE
Detector
Dual detectors; simultaneous dual wavelength
Optical System
Filter-based; preconfigured modules
Light Source
UV Xenon flash lamp; Optional TRF Laser
Excitation and Emission Filters
Preconfigured filter modules (e.g., 1001, 2001, etc.)
MASS ANALYZER
Autofocus
Not specified
Plate Shaking
Linear, orbital, double orbital
CHROMATOGRAPHY AND SAMPLE HANDLING
Microplate Formats
1–1536 well plates
Reagent Injector System
Not specified
Plate Reader Mode
Top/Bottom read for FI, TRF, LUM; On-the-fly mode supported
DATA ACQUISITION AND ANALYSIS
Data Acquisition
Kaleido™ software; 10–550 ms read times
Data Analysis
Optional MyAssays® Desktop software
AUTOMATION AND SOFTWARE
Automation
Fully automatable; robotic integration; plate stackers optional
Software
Kaleido™ 4.1; Win10 IoT; Enhanced Security optional
OPERATIONAL FEATURES
Ease of Use
Simplified workflows; preconfigured filters; protocol presets
Maintenance and Durability
Robust; freely configurable/upgradable; >20 yrs proven performance
Operating Conditions
Temp control: 15–65°C heating, 20–25°C ambient
Power and Cooling
100–240 V AC, 50/60 Hz; Cooling not specified
Dimensions and Footprint
446 × 576 × 485 mm; Weight: 90 kg
GENERAL INFORMATION
Brand and Model
EnVision® Nexus™, Revvity
Documentation and Support
Not specified
Compatibility and Connectivity
LAN/Ethernet; HTS integration; certified for HTRF®
Configuration and Options
US-LUM, TRF Laser, Alpha HTS/Enhanced, stackers, filters, secure SW
Licensing and Certification
HTRF® certified; 21 CFR Part 11 compliance capable
Cost and Operational Expenses
Not specified