Thermo Fisher Scientific Varioskan™ LUX Multimode Microplate Reader Catalog no-VLBLATD1

Reading Accuracy

200–399 nm: ±0.003 Abs or ±2%; 400–1000 nm: ±0.003 Abs or ±1%

Absorbance Range

0–6 Abs (200–1000 nm); Linearity: ±2% (96-well: 0–4 Abs, 384-well: 0–3 Abs)

Sensitivity

FI top: <0.4 fmol; FI bottom: <4 fmol; TRF: <1 amol; Lum: <7 amol; Alpha: <100 amol

Measurement Speed

96-well: 15 s; 384-well: 45 s; 1536-well: 135 s; Spectral scan: <2.2 s/well

Dynamic Range

FI top: >6 decades; FI bottom: >5.5 decades; TRF: >6; Lum: >7

Wavelength Range

Abs: 200–1000 nm; FI Ex: 200–822 nm, Em: 270–840 nm; TRF: Ex 334, Em 400–700; Lum: 360–670; Alpha: Ex 680, Em 400–660

Wavelength Selection

Abs/Spectral: Double Monochromator; FI: Dual Mono; Alpha/TRF: Filter

Measurement Technology

Absorbance, FI (incl. FRET), Luminescence, AlphaScreen, TRF (incl. TR-FRET/hTRF)

Detector

PMT

Optical System

Filter and monochromator optics

Light Source

Xenon Flash Lamp, LED

Excitation and Emission Filters

Fixed Ex Filters: Alpha (680 nm), TRF (334 nm); Em Filters purchasable

Autofocus

Not specified

Plate Shaking

Orbital

Microplate Formats

6–1536 wells (Abs: 6–384 wells); µDrop Plates supported

Reagent Injector System

1 or 2 dispensers; 2–5000 µl, 1 µl steps; Simultaneous dispense/measure

Plate Reader Mode

Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra

Data Acquisition

Auto-save to DB to prevent loss

Data Analysis

SkanIt software; Real-time data; Built-in calculations; Export: XLSX, PDF, XML, TXT

Automation

Robot compatible; Dynamic range auto-selection; Auto calibration; Diagnostics

Software

SkanIt (PC); Editions: Research, Drug Discovery; License-free per user

Ease of Use

Smart safety controls; Virtual Pipetting Tool; User-friendly interface

Maintenance and Durability

Auto calibration and self-diagnostics; Long-term durability not specified

Operating Conditions

4°C above ambient to 45°C; CO2/O2 control (range not specified)

Power and Cooling

Not specified

Dimensions and Footprint

58×53×51 cm; 58 kg (23×21×20 in; 128 lb)

Brand and Model

Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader

Documentation and Support

Docs, Certificates, Contact, Order Tracking available

Compatibility and Connectivity

Robot compatible; PC software interface; HTRF & DLReady certified

Configuration and Options

Modular; Configurable techs, reading modes, dispensers; Filters optional

Licensing and Certification

HTRF (CisBio); DLReady (Promega); License-free software

Cost and Operational Expenses

Not specified

Revvity EnVision XCite Multimode Plate Reader

Reading Accuracy

Abs @ 2 OD: <2%; Precision @ 2 OD: <0.1%

Absorbance Range

0–4 OD (200 µL & 50 µL); 0–3 OD (7.5 µL)

Sensitivity

Fluor (Filter): <4 pM; Mono: <10 pM; TRF: <55 fM; TRF Laser: <15 fM; Alpha: <100 amol; Luminescence: <5 amol (US)

Measurement Speed

Kinetic: 6–20 s; Single read: 22–36 s; Full plate times vary by assay and format (5 s–28 min)

Dynamic Range

Same as Absorbance Range

Wavelength Range

Absorbance: 230–1000 nm; Fluorescence: 230–850 nm

Wavelength Selection

Tunable in 0.1 nm increments (both absorbance and fluorescence)

Measurement Technology

Multimode: Absorbance, Fluorescence, TRF, Alpha, Luminescence, Fluorescence Polarization (filter/mono/laser options)

Detector

High-speed detector

Optical System

Hybrid (Filter + Monochromator); Modular mirror design

Light Source

Xenon flash lamp

Excitation and Emission Filters

User-changeable, barcode ID; includes full filter list and recommendations

Autofocus

Not specified

Plate Shaking

Linear, Orbital, Dual Orbital

Microplate Formats

1–3456 wells

Reagent Injector System

Optional 2-channel; 2–475 µL, 1 µL steps; pump speed 100–500 µL/s

Plate Reader Mode

Multimode

Data Acquisition

Times per mode/plate format provided; no detailed process

Data Analysis

Signals VitroVivo software

Automation

Compatible with multiple systems; robotic integration supported

Software

Signals VitroVivo; Enhanced Security option (21 CFR Part 11)

Ease of Use

User-changeable optics; barcode ID; automation compatible

Maintenance and Durability

Minimal downtime; global support

Operating Conditions

2 °C above ambient to 55 °C; <10 min to 37 °C

Power and Cooling

100–240V, 50/60 Hz; Cooling: Not specified

Dimensions and Footprint

H: 580 mm; D: 550 mm; W: 420 mm; Weight: 50 kg

Brand and Model

EnVision™ XCite multimode plate reader

Documentation and Support

Global network of >300 support staff; filter/dye support

Compatibility and Connectivity

Automation systems; barcode reader; CAN card interface

Configuration and Options

Fully upgradeable; Optional: Alpha, Mono, Laser TRF, US Luminescence, Quad mono, injectors, stackers, Enhanced Security

Licensing and Certification

21 CFR Part 11 support; SelectScience® Seal of Quality; For research use only

Cost and Operational Expenses

Not specified