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$WH6TLPERFORMANCE
Reading Accuracy
Absorbance Range
Sensitivity
Measurement Speed
Dynamic Range
MASS RANGE AND ANALYTE DETECTION
Wavelength Range
Wavelength Selection
Measurement Technology
IONIZATION, SOURCE AND INTERFACE
Detector
Optical System
Light Source
Excitation and Emission Filters
MASS ANALYZER
Autofocus
Plate Shaking
CHROMATOGRAPHY AND SAMPLE HANDLING
Microplate Formats
Reagent Injector System
Plate Reader Mode
DATA ACQUISITION AND ANALYSIS
Data Acquisition
Data Analysis
AUTOMATION AND SOFTWARE
Automation
Software
OPERATIONAL FEATURES
Ease of Use
Maintenance and Durability
Operating Conditions
Power and Cooling
Dimensions and Footprint
GENERAL INFORMATION
Brand and Model
Documentation and Support
Compatibility and Connectivity
Configuration and Options
Licensing and Certification
Cost and Operational Expenses
Moleculer Devices SpectraMax iD5 Multi-Mode Microplate Readers
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$W7FTLPERFORMANCE
Reading Accuracy
< ±0.010 OD ±1.0% (Accuracy), < ±0.003 OD ±1.0% (Precision), 0–3 OD (VIS/UV)
Absorbance Range
0–3 OD (VIS & UV)
Sensitivity
FL (Mono): 1 pM; FL (Filters): 0.3–0.5 pM; Lumi: 2–4 pM; TRF: 10 fM; FP: 1–2 mP @ 1–10 nM
Measurement Speed
Abs: 28s; FL: 21s; Lumi: 21s; TRF: 25s; FP: 35s
Dynamic Range
Wide (due to ultra-cooled PMT)
MASS RANGE AND ANALYTE DETECTION
Wavelength Range
Abs: 230–1000 nm; FL Ex: 250–830 nm; FL Em: 270–850 nm; Lumi: 300–850 nm; TRF: Ex 350 nm, Em 450–750 nm; FP: 300–750 nm
Wavelength Selection
Hybrid (Monochromator + Filters), 1-nm steps, NFC filter ID
Measurement Technology
Hybrid Multi-Mode (Abs, FL, Lumi, TRF, FP, expandable to TR-FRET, BRET, etc.)
IONIZATION, SOURCE AND INTERFACE
Detector
Silicon photodiode, ultra-cooled PMT
Optical System
Hybrid system, xenon flash lamp, ultra-cooled PMT
Light Source
High-power xenon flash lamp
Excitation and Emission Filters
Hybrid system, auto ID via NFC
MASS ANALYZER
Autofocus
Not specified
Plate Shaking
Linear, Orbital, Double orbital
CHROMATOGRAPHY AND SAMPLE HANDLING
Microplate Formats
6–384 well plates
Reagent Injector System
SpectraMax Injector w/ SmartInject® (low dead volume, overflow protection)
Plate Reader Mode
Abs, FL (top/bottom), Lumi (top/bottom), TRF, FP, HTRF; Expandable to TR-FRET, BRET, DLR, WB
DATA ACQUISITION AND ANALYSIS
Data Acquisition
Onboard storage, QuickSync to network, Excel-compatible output
Data Analysis
SoftMax® Pro / Pro GxP Software
AUTOMATION AND SOFTWARE
Automation
Robotics / automation supported
Software
SoftMax® Pro, Pro 7.2 GxP, Touch Software
OPERATIONAL FEATURES
Ease of Use
Touchscreen, NFC, Quick Read, protocol library, temp control, auto filter ID
Maintenance and Durability
Not specified
Operating Conditions
Ambient +5°C to 66°C
Power and Cooling
Ultra-cooled PMT; power specs not specified
Dimensions and Footprint
H: 40.1 cm; W: 53.2 cm; D: 59.8 cm
GENERAL INFORMATION
Brand and Model
Moleculer Devices, SpectraMax® iD5
Documentation and Support
Validation plates, GxP Validation Package, installation/training services
Compatibility and Connectivity
QuickSync, network integration, touchscreen/computer operation
Configuration and Options
iD5 (Abs/FL/Lumi/TRF/FP + expansions)
Licensing and Certification
GxP-compliant (SoftMax® Pro 7.2 GxP, Starter Kit), FDA 21 CFR Part 11, EudraLex Annex 11
Cost and Operational Expenses
Not specified
Thermo Fisher Scientific Varioskan™ LUX Multimode Microplate Reader Catalog no-VLBL00GD1
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$6S3XNPERFORMANCE
Reading Accuracy
0.003 Abs or ±2% (200–399 nm), ±1% (400–1000 nm); SD <0.001 Abs, CV <0.5% @ 450 nm
Absorbance Range
0–6 Abs; Linearity: 0–4 Abs (96-well), 0–3 Abs (384-well) @ 450 nm
Sensitivity
< 0.4 fmol fluorescein/well; < 7 amol ATP/well
Measurement Speed
15s (96-well), 45s (384-well), 135s (1536-well); < 2.2s/well spectral scan
Dynamic Range
Fluorescence > 6 decades; Luminescence > 7 decades; auto range selection
MASS RANGE AND ANALYTE DETECTION
Wavelength Range
Abs: 200–1000 nm; Fluor Ex: 200–822 nm, Em: 270–840 nm; Lumi: 360–670 nm
Wavelength Selection
Double monochromators; filters for TRF
Measurement Technology
Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen, TRF
IONIZATION, SOURCE AND INTERFACE
Detector
Photomultiplier Tube (PMT)
Optical System
Filter and monochromator optics
Light Source
Xenon Flash Lamp
Excitation and Emission Filters
Emission filters available; filters used in TRF
MASS ANALYZER
Autofocus
Not specified
Plate Shaking
Orbital
CHROMATOGRAPHY AND SAMPLE HANDLING
Microplate Formats
6–1536-well plates; Absorbance: up to 384-well; μDrop compatible
Reagent Injector System
0, 1, or 2 dispensers; 2–5000 μL in 1 μL steps; supports simultaneous dispense
Plate Reader Mode
Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra
DATA ACQUISITION AND ANALYSIS
Data Acquisition
Continuous save; real-time spectra/kinetics display
Data Analysis
Built-in calculations; export: *.xlsx, *.pdf, *.xml, *.txt
AUTOMATION AND SOFTWARE
Automation
Auto range selection; auto calibration; self-diagnostics
Software
SkanIt Software; PC interface; license free; includes virtual pipetting tool
OPERATIONAL FEATURES
Ease of Use
Intuitive UI; safety controls; simplified setup
Maintenance and Durability
Auto calibration; self-diagnostics; designed for reliability
Operating Conditions
Temp: Ambient +4° to 45°C; CO₂: 0.1–15%; O₂: 1–21%
Power and Cooling
Not specified
Dimensions and Footprint
58 × 53 × 51 cm; 57 kg
GENERAL INFORMATION
Brand and Model
Thermo Scientific Varioskan LUX
Documentation and Support
Docs & certificates available; contact support
Compatibility and Connectivity
PC software interface; Connect: Lab, Data, Apps
Configuration and Options
Modular; top/bottom read; 0–2 dispensers; filters sold separately
Licensing and Certification
HTRF Certified; DLReady validated; 21 CFR Part 11 (Drug Discovery Edition)
Cost and Operational Expenses
Not specified