Shimadzu Nexera Mikros Triple Quadrupole LC-MS/MS

Mass Accuracy

±0.1 Da (depending on calibration)

Resolution

> 0.7 Da (depending on MS mode)

Sensitivity

High Bioanalytical Sensitivity

Speed

Minimized System Volume & Gradient Delay: Achieved with the SIL-40C XR autosampler’s programmable loop cut-off.

Efficient Sample Handling: A high-pressure valve diverts the sample loop once the needle is drained.

Key Benefit: Reduces microflow analysis run times.

Dynamic Range

0.00763 – 62.5 µg/mL for the analysis of trastuzumab-derived peptides.

Mass Range

10 to 2,000 m/z

Detection Limit

Lower Limit of Detection (LOD): 0.025 µg/mL for signature peptides from the Fab region of antibodies.

Enhanced Sensitivity: Shim-pack MC PLONAS 2.7 μm columns achieve lower LODs and LOQs.

Detector

Triple quadrupole mass spectrometer

Ionization Method

Electrospray ionization (ESI)

Ion Source

Shimadzu Micro-ESI™ LCMS Source: Optimized for precision and sensitivity.

Available Options:

• Micro-ESI 8060: Compatible with LCMS-8045/8050/8060(NX) systems.


• Micro-ESI: Standard option for versatile applications.

Interface

Micro-ESI

Gas Flow and Requirements

Nitrogen (N2): 99.999%, Argon (Ar) for collision gas.

Mass Analyzer

Triple quadrupole

Quadrupole

UF-Qarray™ II

Chromatography

Microflow liquid chromatography.

The Nexera Mikros uses flow rates from 1 to 500 μL/min and column I.D. from 0.1 to 1 mm.

Sample Handling

The system can be used with a trap and elute system, ideal for analyses with larger injection volumes or when some degree of sample cleanup is desirable.

Injection System

SIL-40C XR autosampler.

Injection volume range from 0.1 to 50 μL and uses a total injection method.

Data Acquisition

Continuous (full scan, SIM, MRM)

Data Analysis

MRM measurement is used to quantify Fab-derived peptides.

Automation

Fully automated sample injection and analysis.

Software

LabSolutions LCMS, LabSolutions CR, LabSolutions MS.

Ease of Use

Shimadzu Micro-ESI LCMS Source: Designed for optimal sensitivity and ease of use.

Easy Adjustment: X–Y adjustable stage & viewing camera for precise ESI spray needle positioning.

UF-Link Column Installation: CTO-Mikros oven's snap-in locking system ensures correct microflow column setup without disrupting needle position.

Maintenance and Durability

The LCMS-8060NX with the new ion guide UF-Qarray™ II and the UF-Lens™ II increases the robustness and minimizes downtime of the instrument.

Operating Conditions

10°C to 40°C (ambient temperature)

Power and Cooling

100–240V, 50/60 Hz; Air cooling.

Dimensions and Footprint

510 mm (W) x 490 mm (D) x 510 mm (H)

Brand and Model

Shimadzu Nexera Mikros LC-MS/MS

Documentation and Support

Shimadzu Global Support, Online manuals, FAQs.

Compatibility and Connectivity

USB, Ethernet, LAN, Wi-Fi, MS data compatibility.

Configuration and Options

Dual ion source, Various detector configurations.

Licensing and Certification

CE, RoHS, ISO 9001.

Cost and Operational Expenses

Varies based on configuration, operational cost depends on sample throughput and consumables used.