Moleculer Devices SpectraMax iD3 Multi-Mode Microplate Readers

Reading Accuracy

< ±0.010 OD ±1.0%; Precision: < ±0.003 OD ±1.0% (0–3 OD VIS/UV)

Absorbance Range

0–3 OD (VIS & UV)

Sensitivity

Fluorescence: 1 pM fluorescein (96/384 wells); Luminescence: 2 pM ATP (96), 4 pM (384)

Measurement Speed

Abs: 28 sec; Fluor: 21 sec; Lum: 21 sec

Dynamic Range

Not specified

Wavelength Range

Abs: 230–1000 nm; Fluor Ex: 250–830 nm; Em: 270–850 nm; Lum: 300–850 nm

Wavelength Selection

Monochromator, 1-nm increments

Measurement Technology

Xenon flash lamp, silicon photodiode, ultra-cooled PMT

Detector

Silicon photodiode, ultra-cooled PMT

Optical System

Not specified

Light Source

High-power xenon flash lamp

Excitation and Emission Filters

Not specified (uses monochromator)

Autofocus

Not specified

Plate Shaking

Linear, Orbital, Double orbital

Microplate Formats

6–384 well plates

Reagent Injector System

Present

Plate Reader Mode

Absorbance, Fluorescence (top/bottom), Luminescence (top)

Data Acquisition

Endpoint, Kinetic, Spectrum scan, Well scan

Data Analysis

SoftMax® Pro GxP Software

Automation

Robotics/automation supported

Software

SoftMax® Pro GxP

Ease of Use

Not specified

Maintenance and Durability

Not specified

Operating Conditions

Temp: Ambient +5°C to 66°C

Power and Cooling

Ultra-cooled PMT; other power specs not specified

Dimensions and Footprint

H: 40.1 cm; W: 53.2 cm; D: 59.8 cm

Brand and Model

Moleculer Devices, SpectraMax® iD3

Documentation and Support

Not specified

Compatibility and Connectivity

Robotics/automation support; other compatibility not specified

Configuration and Options

Not specified

Licensing and Certification

GxP-compliant software (SoftMax® Pro GxP); certifications not specified

Cost and Operational Expenses

Not specified

Thermo Fisher Scientific Varioskan™ LUX Multimode Microplate Reader Catalog no-VLB000D0

Reading Accuracy

±0.003 Abs or ±2% (200–399 nm); ±0.003 Abs or ±1% (400–1000 nm); SD < 0.001 Abs; CV < 0.5% @ 450 nm

Absorbance Range

0–6 Abs

Sensitivity

<0.4 fmol (top), <4 fmol (bottom), fluorescein/well

Measurement Speed

15 s (96-well), 45 s (384-well), 135 s (1536-well), <2.2 s/well scan

Dynamic Range

>6 decades (top), >5.5 decades (bottom)

Wavelength Range

Abs: 200–1000 nm; Exc: 200–822 nm; Em: 270–840 nm

Wavelength Selection

Double monochromators (Abs & Fluorescence)

Measurement Technology

Absorbance, Fluorescence, Luminescence, AlphaScreen/LISA, TRF

Detector

Photomultiplier Tube (PMT)

Optical System

Filter + monochromator optics

Light Source

Xenon Flash Lamp

Excitation and Emission Filters

Monochromators + optional filters available

Autofocus

Not specified

Plate Shaking

Orbital

Microplate Formats

6–1536 wells, μDrop; Abs: up to 384-well

Reagent Injector System

Optional: 0, 1, or 2 dispensers; supports simultaneous dispensing

Plate Reader Mode

Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra

Data Acquisition

Continuous data saving

Data Analysis

Built-in calculations, real-time display, one-click export, QC tools

Automation

Auto range selection, calibration, self-diagnostics; automation-compatible

Software

SkanIt (PC), Research & Drug Discovery Editions, virtual pipetting

Ease of Use

Auto setup, smart safety controls, intuitive software

Maintenance and Durability

Auto calibration, self-diagnostics, high reliability

Operating Conditions

Ambient +4°C to 45°C, optional gas control module

Power and Cooling

100/240 V, 50/60 Hz; cooling not specified

Dimensions and Footprint

58×53×51 cm (23×21×20 in), 54 kg (119 lb)

Brand and Model

Thermo Scientific Varioskan LUX

Documentation and Support

Available; contact sales for more info

Compatibility and Connectivity

PC Software, Automation-compatible, HTRF & DLReady certified

Configuration and Options

Modular; various tech, reading modes, dispensers; optional gas module

Licensing and Certification

SkanIt free license; Drug Discovery: 21 CFR Part 11, HTRF, DLReady

Cost and Operational Expenses

Not specified

Revvity EnVision Nexus Multimode Microplate Reader

Reading Accuracy

Abs: <0.05% at 0.5 OD; Precision: <0.01% at 0.5 OD

Absorbance Range

0–4 OD (50 µL)

Sensitivity

FI: <2 pM; FP: <1.15 mP SD; TRF: <0.02 pM; TRF Laser: <0.004 pM; Alpha HTS: 0.2 ng/mL; Alpha Enhanced: 0.4 ng/mL; Lum: <0.25 pM; Enhanced Lum: <0.025 pM; US Lum: <0.001 pM

Measurement Speed

384-well FI: 13 sec (typical); Max: 14 sec; US-LUM: 00:40

Dynamic Range

Not specified

Wavelength Range

Not specified

Wavelength Selection

Filter-based; assay-ready preconfigured filters

Measurement Technology

Abs, FI, FP, TRF, TRF Laser, Lum, US-LUM, Alpha HTS/Enhanced

Detector

Dual detectors; simultaneous dual wavelength

Optical System

Filter-based; preconfigured modules

Light Source

UV Xenon flash lamp; Optional TRF Laser

Excitation and Emission Filters

Preconfigured filter modules (e.g., 1001, 2001, etc.)

Autofocus

Not specified

Plate Shaking

Linear, orbital, double orbital

Microplate Formats

1–1536 well plates

Reagent Injector System

Not specified

Plate Reader Mode

Top/Bottom read for FI, TRF, LUM; On-the-fly mode supported

Data Acquisition

Kaleido™ software; 10–550 ms read times

Data Analysis

Optional MyAssays® Desktop software

Automation

Fully automatable; robotic integration; plate stackers optional

Software

Kaleido™ 4.1; Win10 IoT; Enhanced Security optional

Ease of Use

Simplified workflows; preconfigured filters; protocol presets

Maintenance and Durability

Robust; freely configurable/upgradable; >20 yrs proven performance

Operating Conditions

Temp control: 15–65°C heating, 20–25°C ambient

Power and Cooling

100–240 V AC, 50/60 Hz; Cooling not specified

Dimensions and Footprint

446 × 576 × 485 mm; Weight: 90 kg

Brand and Model

EnVision® Nexus™, Revvity

Documentation and Support

Not specified

Compatibility and Connectivity

LAN/Ethernet; HTS integration; certified for HTRF®

Configuration and Options

US-LUM, TRF Laser, Alpha HTS/Enhanced, stackers, filters, secure SW

Licensing and Certification

HTRF® certified; 21 CFR Part 11 compliance capable

Cost and Operational Expenses

Not specified

Thermo Fisher Scientific Varioskan™ LUX Multimode Microplate Reader Catalog no-VLBLATD0

Reading Accuracy

0.003 Abs or ±2% (200–399 nm); ±1% (400–1000 nm)

Absorbance Range

0–6 Abs; 200–1000 nm

Sensitivity

FI Top: <0.4 fmol; FI Bottom: <4 fmol; TRF: <1 amol; Lum: <7 amol; AS: <100 amol

Measurement Speed

15s (96-well), 45s (384-well), 135s (1536-well); <2.2s/well scan (400–500 nm)

Dynamic Range

FI Top: >6 decades; FI Bottom: >5.5; Lum: >7 decades; Auto range selection

Wavelength Range

Abs: 200–1000 nm; FI: Ex 200–822, Em 270–840; TRF: Ex 334, Em 400–700; Lum: 360–670; AS: Ex 680, Em 400–660

Wavelength Selection

Double Monochromator (Abs/FI); Filters (TRF/AS); Filter + Monochromator Optics

Measurement Technology

Absorbance, FI (incl. FRET), Luminescence, AlphaScreen, TRF (incl. TR-FRET)

Detector

Photomultiplier Tube (PMT)

Optical System

Filter and monochromator optics

Light Source

Xenon Flash Lamp, LED

Excitation and Emission Filters

Fixed and purchasable filters (TRF: 334 nm, AS: 680 nm)

Autofocus

Not specified

Plate Shaking

Orbital

Microplate Formats

6–1536-well; Absorbance: 6–384-well; μDrop plates supported

Reagent Injector System

0 (standard); models with 1 or 2; simultaneous dispense + read supported

Plate Reader Mode

Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra

Data Acquisition

Continuous database saving

Data Analysis

Built-in calc: blank sub, SD/CV, curves, dose response, QC, classification

Automation

Robot compatible; Auto calibration; Self-diagnostics

Software

SkanIt Software (PC); Research & Drug Discovery Editions; license-free

Ease of Use

Auto range selection, Virtual Pipetting Tool, smart controls, real-time data

Maintenance and Durability

Auto calibration & diagnostics; durability not specified

Operating Conditions

Temp: +4° to 45°C; CO₂: 0.1–15%; O₂: 1–21%

Power and Cooling

Not specified

Dimensions and Footprint

58×53×51 cm / 23×21×20 in; Weight: 57 kg / 125 lb

Brand and Model

Thermo Scientific™ Varioskan™ LUX Multimode Microplate Reader

Documentation and Support

Docs & Certificates available; Contact Us options

Compatibility and Connectivity

PC Software, Robot compatible; HTRF & DLReady certified

Configuration and Options

Modular; configurable tech, read type, injectors

Licensing and Certification

License-free; HTRF and DLReady validated

Cost and Operational Expenses

Not specified