Moleculer Devices SpectraMax iD5 Multi-Mode Microplate Readers

Reading Accuracy

< ±0.010 OD ±1.0% (Accuracy), < ±0.003 OD ±1.0% (Precision), 0–3 OD (VIS/UV)

Absorbance Range

0–3 OD (VIS & UV)

Sensitivity

FL (Mono): 1 pM; FL (Filters): 0.3–0.5 pM; Lumi: 2–4 pM; TRF: 10 fM; FP: 1–2 mP @ 1–10 nM

Measurement Speed

Abs: 28s; FL: 21s; Lumi: 21s; TRF: 25s; FP: 35s

Dynamic Range

Wide (due to ultra-cooled PMT)

Wavelength Range

Abs: 230–1000 nm; FL Ex: 250–830 nm; FL Em: 270–850 nm; Lumi: 300–850 nm; TRF: Ex 350 nm, Em 450–750 nm; FP: 300–750 nm

Wavelength Selection

Hybrid (Monochromator + Filters), 1-nm steps, NFC filter ID

Measurement Technology

Hybrid Multi-Mode (Abs, FL, Lumi, TRF, FP, expandable to TR-FRET, BRET, etc.)

Detector

Silicon photodiode, ultra-cooled PMT

Optical System

Hybrid system, xenon flash lamp, ultra-cooled PMT

Light Source

High-power xenon flash lamp

Excitation and Emission Filters

Hybrid system, auto ID via NFC

Autofocus

Not specified

Plate Shaking

Linear, Orbital, Double orbital

Microplate Formats

6–384 well plates

Reagent Injector System

SpectraMax Injector w/ SmartInject® (low dead volume, overflow protection)

Plate Reader Mode

Abs, FL (top/bottom), Lumi (top/bottom), TRF, FP, HTRF; Expandable to TR-FRET, BRET, DLR, WB

Data Acquisition

Onboard storage, QuickSync to network, Excel-compatible output

Data Analysis

SoftMax® Pro / Pro GxP Software

Automation

Robotics / automation supported

Software

SoftMax® Pro, Pro 7.2 GxP, Touch Software

Ease of Use

Touchscreen, NFC, Quick Read, protocol library, temp control, auto filter ID

Maintenance and Durability

Not specified

Operating Conditions

Ambient +5°C to 66°C

Power and Cooling

Ultra-cooled PMT; power specs not specified

Dimensions and Footprint

H: 40.1 cm; W: 53.2 cm; D: 59.8 cm

Brand and Model

Moleculer Devices, SpectraMax® iD5

Documentation and Support

Validation plates, GxP Validation Package, installation/training services

Compatibility and Connectivity

QuickSync, network integration, touchscreen/computer operation

Configuration and Options

iD5 (Abs/FL/Lumi/TRF/FP + expansions)

Licensing and Certification

GxP-compliant (SoftMax® Pro 7.2 GxP, Starter Kit), FDA 21 CFR Part 11, EudraLex Annex 11

Cost and Operational Expenses

Not specified

Thermo Fisher Scientific Varioskan™ LUX Multimode Microplate Reader Catalog no-VLBLATGD1

Reading Accuracy

±2% (200–399 nm), ±1% (400–1000 nm); Precision: SD <0.001 Abs; Linearity: ±2% (0–4 Abs at 450 nm)

Absorbance Range

0–6 Abs; 200–1000 nm

Sensitivity

Fluorescence (top): <0.4 fmol; Luminescence: <7 amol; AlphaScreen: <100 amol

Measurement Speed

96-well: 15s; 384-well: 45s; 1536-well: 135s; Scan: <2.2s/well

Dynamic Range

Fluorescence: >6 decades; Luminescence: >7 decades; Auto range selection

Wavelength Range

Abs: 200–1000 nm; Fluo Ex: 200–822 nm; Fluo Em: 270–840 nm; TRF: 334/400–700 nm; Lum: 360–670 nm; AS: 680/400–660 nm

Wavelength Selection

Double Monochromator (Abs & Fluo); Filter (TRF & AlphaScreen)

Measurement Technology

Absorbance, Fluorescence Intensity, Luminescence, TRF, AlphaScreen

Detector

Photomultiplier Tube (PMT)

Optical System

Filter & Monochromator optics

Light Source

Xenon Flash Lamp, LED

Excitation and Emission Filters

Excitation: Fixed; Emission: Available for purchase

Autofocus

Not specified

Plate Shaking

Orbital

Microplate Formats

6–1536-well; μDrop plates

Reagent Injector System

0, 1, or 2 dispensers; 2–5000 μl in 1 μl increments

Plate Reader Mode

Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra

Data Acquisition

Continuous save to database

Data Analysis

Built-in calc: blank subtraction, stats, standard curve, kinetics, spectra, etc.

Automation

Auto calibration, diagnostics, safety controls, robot compatible

Software

SkanIt Software, license-free; Editions: Research, Drug Discovery

Ease of Use

Auto range select, virtual pipetting, simplified setup & export

Maintenance and Durability

Auto calibration, self-diagnostics

Operating Conditions

4° above ambient to 45°C; CO₂: 0.1–15%; O₂: 1–21%

Power and Cooling

Not specified

Dimensions and Footprint

58×53×51 cm (23×21×20 in); Weight: 57 kg (125 lb)

Brand and Model

Thermo Scientific™ Varioskan™ LUX

Documentation and Support

Docs & certificates available; sales support

Compatibility and Connectivity

Robot compatible; SkanIt PC Software

Configuration and Options

Modular & upgradable; emission filters purchasable

Licensing and Certification

HTRF™ certified; DLReady™ validated; FDA 21 CFR Part 11 compliant (Drug Discovery Edition)

Cost and Operational Expenses

Not specified

Thermo Fisher Scientific Varioskan™ LUX Multimode Microplate Reader Catalog no-VLBL0TD1

Reading Accuracy

0.003 Abs or ±2% (200–399 nm), ±1% (400–1000 nm), SD < 0.001 Abs at 450 nm

Absorbance Range

0–6 Abs, Linearity ±2% (0–4 Abs 96-well), ±2% (0–3 Abs 384-well)

Sensitivity

FI Top: <0.4 fmol; FI Bottom: <4 fmol; TRF: <1 amol; Lum: <7 amol

Measurement Speed

15s (96-well), 45s (384-well), 135s (1536-well), Spectral scan <2.2s/well

Dynamic Range

FI Top: >6 decades; FI Bottom: >5.5 decades; TRF: >6; Lum: >7

Wavelength Range

Abs: 200–1000 nm; FI: Ex 200–822, Em 270–840; TRF: Ex 334, Em 400–700

Wavelength Selection

Double Monochromator (Abs), Double Mono (FI), Filters (TRF)

Measurement Technology

Absorbance, FI, Luminescence, TRF, AlphaScreen/AlphaLISA

Detector

Photomultiplier Tube (PMT)

Optical System

Filter & Monochromator

Light Source

Xenon Flash Lamp

Excitation and Emission Filters

TRF Excitation: 334 nm (fixed); Emission filters available

Autofocus

Not specified

Plate Shaking

Orbital

Microplate Formats

6 to 1536-well; Abs: up to 384-well; μDrop supported

Reagent Injector System

0–2 dispensers; Volume: 2–5000 µl; 1 µl increments

Plate Reader Mode

Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra

Data Acquisition

Continuous save to database

Data Analysis

Built-in: blank subtraction, stats, curves, QC, formulas

Automation

Auto range selection, calibration, self-diagnostics

Software

SkanIt PC Software, Research & Drug Discovery Editions

Ease of Use

Intuitive UI, Virtual Pipetting Tool, Smart Safety Controls

Maintenance and Durability

Auto calibration, self-diagnostics; durability not specified

Operating Conditions

Incubator: Ambient +4° to 45°C; 50/60 Hz; 100–240 V

Power and Cooling

Voltage: 100–240 V; Hz: 50/60; Cooling: not specified

Dimensions and Footprint

58×53×51 cm (23×21×20 in); Weight: 58 kg (128 lb)

Brand and Model

Thermo Scientific™ Varioskan™ LUX

Documentation and Support

Docs & certificates listed; contact available; detail not specified

Compatibility and Connectivity

PC software; HTRF & DLReady certified; other connections not specified

Configuration and Options

Modular, 0–2 dispensers, top/bottom read, filter options

Licensing and Certification

License-free; HTRF™ and DLReady™ validated

Cost and Operational Expenses

Not specified

Thermo Fisher Scientific Varioskan™ LUX Multimode Microplate Reader Catalog no-VLBL00GD1

Reading Accuracy

0.003 Abs or ±2% (200–399 nm), ±1% (400–1000 nm); SD <0.001 Abs, CV <0.5% @ 450 nm

Absorbance Range

0–6 Abs; Linearity: 0–4 Abs (96-well), 0–3 Abs (384-well) @ 450 nm

Sensitivity

< 0.4 fmol fluorescein/well; < 7 amol ATP/well

Measurement Speed

15s (96-well), 45s (384-well), 135s (1536-well); < 2.2s/well spectral scan

Dynamic Range

Fluorescence > 6 decades; Luminescence > 7 decades; auto range selection

Wavelength Range

Abs: 200–1000 nm; Fluor Ex: 200–822 nm, Em: 270–840 nm; Lumi: 360–670 nm

Wavelength Selection

Double monochromators; filters for TRF

Measurement Technology

Absorbance, Fluorescence Intensity, Luminescence, AlphaScreen, TRF

Detector

Photomultiplier Tube (PMT)

Optical System

Filter and monochromator optics

Light Source

Xenon Flash Lamp

Excitation and Emission Filters

Emission filters available; filters used in TRF

Autofocus

Not specified

Plate Shaking

Orbital

Microplate Formats

6–1536-well plates; Absorbance: up to 384-well; μDrop compatible

Reagent Injector System

0, 1, or 2 dispensers; 2–5000 μL in 1 μL steps; supports simultaneous dispense

Plate Reader Mode

Endpoint, Kinetic, Spectra, Multipoint, Kinetic Spectra

Data Acquisition

Continuous save; real-time spectra/kinetics display

Data Analysis

Built-in calculations; export: *.xlsx, *.pdf, *.xml, *.txt

Automation

Auto range selection; auto calibration; self-diagnostics

Software

SkanIt Software; PC interface; license free; includes virtual pipetting tool

Ease of Use

Intuitive UI; safety controls; simplified setup

Maintenance and Durability

Auto calibration; self-diagnostics; designed for reliability

Operating Conditions

Temp: Ambient +4° to 45°C; CO₂: 0.1–15%; O₂: 1–21%

Power and Cooling

Not specified

Dimensions and Footprint

58 × 53 × 51 cm; 57 kg

Brand and Model

Thermo Scientific Varioskan LUX

Documentation and Support

Docs & certificates available; contact support

Compatibility and Connectivity

PC software interface; Connect: Lab, Data, Apps

Configuration and Options

Modular; top/bottom read; 0–2 dispensers; filters sold separately

Licensing and Certification

HTRF Certified; DLReady validated; 21 CFR Part 11 (Drug Discovery Edition)

Cost and Operational Expenses

Not specified