Thermofisher Orbitrap Exploris 240 Mass Spectrometer

Mass Accuracy

• <1 ppm RMS drift for at least 5 days measured with FlexMix


• EASY-IC source achieves 3 ppm RMS drift over 24 h with Thermo Scientific Pierce FlexMix calibration solution


• Internal lock mass calibration achieves <1 ppm RMS drift over 24 h with FlexMix

Resolution

Up to 240,000 at m/z 2001234

Sensitivity

• Full Scan: < 3.0 fg reserpine on column


• tMS2: < 3.0 fg reserpine on column


• tSIM: < 1.5 fg reserpine on column


• MS/MS: 200 fg reserpine on column S/N 100:1


• tSIM: 200 fg reserpine on column S/N 250:15

Speed

Up to 30 Hz at resolution setting at 11,250
Up to 22 Hz at resolution setting of 15,000 at m/z 200

Dynamic Range

>5,000 within a single Orbitrap mass analyzer spectrum
>5000:1 within a single Orbitrap mass analyzer spectrum

Mass Range

40 to 6000 m/z12
Standard mass range m/z 40–6,000
Up to m/z 8,000 with BioPharma option

Detection Limit

< 3.0 fg reserpine on column

Detector

Independent Charge Detector

Ionization Method

Electrospray ionization (ESI)
Heated electrospray ionization (H-ESI)

Ion Source

Thermo Scientific™ OptaMax™ NG ion source

Interface

Atmospheric pressure ionization (API) interface

Gas Flow and Requirements

• Source gas: High-purity nitrogen (>99% pure at 600 ± 50 kPa [6.0 ± 0.5 bar, 87 ± 7 psi])


• A pre-regulator may be required to maintain stable source gas pressure.


• HCD collision gas: Ultra-high-purity nitrogen (UHP > 99.999% pure) at 600 ± 50 kPa [6.0 ± 0.5 bar]


• With EASY-IC: Ultra-high-purity nitrogen (UHP > 99.999% pure) at 600 ± 50 kPa [6.0 ± 0.5 bar]

Mass Analyzer

High-Field Orbitrap mass analyzer with 4 kV central electrode voltage
Thermo Scientific™ Orbitrap™ mass analyzer
Orbitrap

Quadrupole

Advanced quadrupole technology (AQT)
Segmented quadrupole mass filter for precursor ion selection with variable precursor isolation width from 0.4 to 2,000 Da

Chromatography

LC-MS

Sample Handling

Automated liquid handling
nanoLC
Sample pre-treatment systems

Injection System

Liquid Chromatography (LC) and Ion Source Options (ESI, APCI)

Data Acquisition

• High-performance PC with Intel® microprocessor


• High-resolution LED color monitor


• Microsoft® Windows® Enterprise (Long Term Service version) OS


• High-speed real-time data acquisition and instrument control


• Automatic calibration of ion transfer and analysis parameters

Data Analysis

Thermo Scientific™ Orbitrap Exploris™ instrument control software
Thermo Scientific™ Xcalibur™ software

Automation

AcquireX data acquisition workflows provide comprehensive small molecule sample profiling on an LC timescale using automated, logic-based sample re-injection with inter-run inclusion and exclusion lists

Software

• Thermo Scientific™ Orbitrap Exploris™ instrument control software


• Thermo Scientific™ Xcalibur™ software


• Thermo Scientific™ Proteome Discoverer™ software


• Thermo Scientific™ BioPharma Finder™ software


• Thermo Scientific™ ProSightPD™ software


• Thermo Scientific™ Compound Discoverer™ software


• Thermo Scientific™ TraceFinder™ software


• Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)

Ease of Use

Next-generation software with intuitive tuning, single-click calibration, drag-and-drop method setup, and pre-defined experiments

Maintenance and Durability

Minimal user intervention for maintenance

Operating Conditions

• Operating temperature: 18–27 °C (64–81 °F)


• Relative humidity: 20–80% (no condensation)


• Indoor use up to 3,000 m (10,000 ft) altitude

Power and Cooling

• 2×208–240 Vac single phase, 15 A, 50/60 Hz, with earth ground for instrument and source vacuum pump


• 208–240 Vac single phase, 15 A, 50/60 Hz, with earth ground for the data system


• System power consumption: 3440 W (11,730 Btu/h) including air conditioning needs

Dimensions and Footprint

534 × 763 × 703 mm (21 × 30 × 27.7 in)
120kg (265 lb.) without data system, vacuum rough pumps and optional items

Brand and Model

Thermo Scientific™, Orbitrap Exploris™ 240 mass spectrometer, Orbitrap Exploris 240 MS

Documentation and Support

Comprehensive manuals, online tutorials, 24/7 technical support, and system updates

Compatibility and Connectivity

Compatible with Thermo Fisher software (Xcalibur, Thermo Scientific Chromeleon) and industry-standard data formats (e.g., mzML)

Configuration and Options

Thermo Scientific BioPharma option enables analysis of large molecules up to m/z 8000 (Request quote)
APCI/APPI probe for OptaMax NG ion source
Thermo Scientific FAIMS Pro Duo interface

Licensing and Certification

For Research Use Only. Not for use in diagnostic procedures.

Cost and Operational Expenses

Approx. $250,000–$350,000 (depending on configuration)
Operational expenses include consumables, maintenance, and software updates