Thermofisher Orbitrap Eclipse™ Tribrid™ Mass Spectrometer

Mass Accuracy

<3 ppm RMS using external calibration, <1 ppm RMS using internal calibration, <1 ppm RMS drift over 24 hours, measured with Flex Mix

Resolution

Up to 500,000 at m/z 200
Optional: Up to 1,000,000 at m/z 200

Sensitivity

• 2 µL of 50 fg/µL reserpine (100 fg total) at 500 µL/min


• Minimum S/N ratio: 200:1


• Transition: m/z 609 → m/z 397, m/z 448


• Unit resolution, full scan MS/MS mode (m/z 165)

Speed

Orbitrap: Up to 40 Hz
Linear Ion Trap: Up to 45 Hz

Dynamic Range

>5000 within a single Orbitrap mass spectrum

Mass Range

Standard: 50 to 2000 m/z
Mid: 200 to 6000 m/z
Optional (HMRn): 500 to 8000 m/z

Detection Limit

Low fmol (femtomolar) range for qualitative and quantitative analysis

Detector

Large surface area ion trap detector

Ionization Method

Heated electrospray ionization (HESI)

Ion Source

Thermo Scientific™ Ion Max NG™ ion source

Interface

API interface consists of a High-Capacity Transfer Tube (HCTT) and an Electrodynamic Ion Funnel (EDIF)

Gas Flow and Requirements

• High-purity Nitrogen (99.5%, 15 L/min) for API source and IRM


• Ultra-high purity Helium (99.999%) with <1 ppm water, oxygen, and total hydrocarbons

Mass Analyzer

Orbitrap1311
Tribrid architecture includes a quadrupole mass filter, dual-pressure linear ion trap and Orbitrap mass analyzers

Quadrupole

QR5 segmented quadrupole mass filter with hyperbolic surfaces

Chromatography

LC-MS

Sample Handling

Automatic sample introduction, compatible with liquid and solid samples

Injection System

Standard and enhanced ionization sources, including ESI, APCI, and more

Data Acquisition

HRAM

Data Analysis

Real-Time Search

Automation

Fully automated analysis, including sample preparation, injection, and data analysis

Software

Thermo Scientific™ Xcalibur™ software15, Orbitrap Eclipse MS instrument control software

Ease of Use

Intuitive, flexible drag-and-drop instrument method editor simplifies method development and creation of unique analytical workflows16
Extensive method template library

Maintenance and Durability

Enhanced Vacuum Technology reduces the pressure in the ultra-high vacuum region to <10-10 Torr17
Stainless steel and aluminum high-vacuum analyzer chambers

Operating Conditions

16–26°C (59–78°F) and relative humidity must be 50–80% with no condensation

Power and Cooling

230 Vac ±10% single phase, 50/60 Hz, with ground, 3x outlets at 15 A each
System averages 2800 W (10,000 Btu/hr) output

Dimensions and Footprint

1270 × 767 × 703 mm (50 × 30.2 × 27.7 in)

Brand and Model

Thermo Scientific™, Orbitrap Eclipse™ Tribrid™ Mass Spectrometer

Documentation and Support

Comprehensive documentation
24/7 technical support, software updates

Compatibility and Connectivity

Compatible with various chromatography systems (LC, GC) and network integration

Configuration and Options

Modular design with multiple ionization sources, collision cells, and detectors

Licensing and Certification

CE certified
RoHS compliant
ISO 9001 certified

Cost and Operational Expenses

High initial investment
Moderate operational expenses for consumables, maintenance contracts optional