Spectral Flow Cytometers

Essential Features

• The se instruments enable high-dimensional analysis of over 45 colors while maintaining outstanding data quality, effortlessly handling both cell-based assays and small particle analysis with exceptional performance.
• With up to 5 lasers, 73 detectors, a proprietary optics design, and advanced electronics, spectral flow cytometry instruments deliver high-resolution, high-sensitivity data across a wide dynamic range for fluorescence detection and particle size measurement. All this while on-board temperature control and integrated sensor circuitry for electronics and fluidics enable real-time status monitoring to ensure consistent, reliable performance in any environment.
• As the first spectral flow cytometer sorters with sort-capable image analysis, these instruments combine spectral flow cytometry with real-time spatial and morphological insights to expand the boundaries of cell analysis and sorting. They enable visual confirmation of cell populations during processing, identification, and isolation of previously undetectable cell types. They also provide streamlined workflows through built-in visual inspection, flexible panel design, and automation.
• The se cell sorters seamlessly integrate performance, safety, and ease of use into both individual labs and core facilities. They offer:

1. Up to 9 lasers and 60 detectors for standard fluorescence or spectral unmixing.
2. Multiple scatter options for simultaneous standard and small particle detection.
3. Flexible sorting formats from tubes to 1536-well plates.
4. A massively parallel architecture that supports complex, high-parameter experiments with up to a 60 × 60 compensation matrix or spectral unmixing without compromising throughput or data quality.

Applications

• Immunophenotyping: Spectral flow cytometry enables detailed profiling of immune cell subsets by detecting numerous surface and intracellular markers simultaneously. This allows identification of rare or novel populations and comprehensive monitoring of immune responses in health and disease.
• Cancer research: The technology facilitates precise characterization of tumor-infiltrating lymphocytes and malignant cells within heterogeneous tumor environments. Its ability to resolve complex marker combinations supports minimal residual disease monitoring, biomarker discovery, and therapy response prediction.
• Infectious disease research: Spectral flow cytometry can capture multiple pathogen-specific immune responses in a single run. This enables differentiation of host immune signatures across infections and provides robust evaluation of vaccine-induced immunity.
• Stem cell and regenerative medicine: The system provides sensitive detection and monitoring of stem and progenitor cells within mixed cell populations. It also supports tracking of differentiation pathways and transplanted cells, advancing regenerative therapy research.
• Extracellular vesicle and small particle analysis: High sensitivity and resolution allow detection and discrimination of exosomes, microvesicles, and even viruses. These capabilities are essential for liquid biopsy applications and the identification of novel biomarkers.
• Extracellular vesicle and small particle analysis: High sensitivity and resolution allow detection and discrimination of exosomes, microvesicles, and even viruses. These capabilities are essential for liquid biopsy applications and the identification of novel biomarkers.
• Clinical diagnostics: Spectral flow cytometry enhances diagnostic accuracy for hematologic malignancies and immune-related disorders. It further supports disease monitoring and assessment of treatment responses in clinical practice.

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