ESI MS

Electrospray Ionization Mass Spectrometry (ESI-MS) is a widely used ionization technique in analytical chemistry, particularly for biomolecules and polar compounds. ESI generates charged droplets from a liquid sample using a high-voltage electric field, leading to solvent evaporation and ion formation. This soft ionization method preserves molecular integrity, making it ideal for analyzing proteins, peptides, nucleotides, and metabolites. ESI-MS is commonly coupled with Liquid Chromatography (LC-MS) for high-throughput analysis in proteomics, pharmaceuticals, and environmental studies. Its ability to produce multiply charged ions enhances mass spectrometric sensitivity, allowing for the detection of large biomolecules with high accuracy and minimal fragmentation.read more