Orbitrap Mass Spectrometry

Essential Features of Orbitrap Mass Spectrometry

• The on-column derivatization GC-MS system features a 2-step injection for rapid derivatization, enables direct analysis without pretreatment, utilizes MBTFA as the derivatizing agent, and is fully compatible with GC-MS systems.
• The Orbitrap mass analyzer and detector streamlines analyses with Chromeleon chromatography data system, offering built-in methods, data processing, and management tools that simplify tasks, reduce errors, and ensure reproducible, high-quality mass monitoring. Its robust performance, rapid calibration, seamless multi-attribute method transfer, high-performance, and integration with workflows and software enable high-throughput and compliant and reliable monitoring of biotherapeutics from development to commercialization.
• The mass spectrometer delivers high-resolution, accurate, and reliable qualitative and quantitative analysis across a mass range of m/z 40–3000, achieving up to 120,000 FWHM resolution and <1 ppm mass accuracy with a single calibration. Designed for operational simplicity and high-throughput performance, it features ready-to-run method templates, a drag-and-drop interface for method development, and advanced capabilities for isotope ratio analysis, preserving intramolecular isotopic information with soft electrospray ionization and high-resolution accurate mass sensitivity.
• The system combines high-resolution GCxGC and ToF-MS with high-resolution deconvolution, enabling users to detect more analytes and confidently identify unknown components in complex samples. Its benchtop design, industry-leading sensitivity, fast acquisition rates up to 200 spectra/second, and ChromaTOF software provide high-quality, deconvoluted spectra and comprehensive two-dimensional separation for efficient and reliable complex sample characterization.
• The cyclic ion-mobility separation (IMS) system combines novel cyclic ion mobility separation with advanced ToF mass spectrometry, enabling separation by both mass-to-charge ratio (m/z) and shape, high-resolution IMSⁿ experiments, and pre-mobility selection of precursor and product ions for unparalleled flexibility in research. Its integration with desorption electrospray ionization (DESI) XS and the latest ToF technology ensures high sensitivity, resolution over 100,000 full width at half maximum (FWHM), accurate low parts-per-million (ppm) mass measurements, and comprehensive structural and spatial characterization of complex samples.

Applications of Orbitrap MS

• Clinical & translational research: Orbitrap HRAM technology enables precise measurement and identification of biomolecules, supporting studies that bridge basic research and patient care. It helps uncover disease mechanisms, identify biomarkers, and facilitate the development of diagnostic and therapeutic strategies.
• Biopharmaceuticals: The Orbitrap mass spectrometry instrumentation is widely used in biopharmaceutical development to analyze protein therapeutics, monitor product quality attributes, and ensure batch-to-batch consistency. Its high accuracy and resolution allow detailed characterization of complex biologics, including monoclonal antibodies and other large biomolecules.
• Food processing contaminants: The technique is applied to detect and quantify contaminants in food products, ensuring safety and regulatory compliance. It can identify chemical residues, additives, and degradation products with high sensitivity and specificity.
• Environmental contaminant analysis: The technology allows precise detection and quantification of pollutants in soil, water, and air samples. High-resolution mass analysis helps trace environmental contaminants, monitor exposure, and support ecological risk assessment.]
• Drugs of abuse identification: Orbitrap HRAM is used in forensic and clinical laboratories to detect and confirm illicit substances in biological samples. Its high sensitivity and mass accuracy allow reliable identification even in complex matrices.
• Antidoping: In sports testing, Orbitrap technology identifies prohibited substances and their metabolites in athletes’ samples. It provides accurate, reproducible results that support anti-doping compliance and fair competition.
• Untargeted metabolomics/lipidomics: The technology allows comprehensive profiling of metabolites and lipids without prior assumptions. It aids in understanding metabolic pathways and studying physiological and pathological changes in biological systems.

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