Agilent 6545XT AdvanceBio LC/Q-TOF
The Agilent 6545XT AdvanceBio LC/Q-TOF provides high-resolution, sensitive analysis for biopharmaceutical applications with precision.
Features
- High-resolution Q-TOF analysis
- Advanced mass spectrometry for biopharmaceuticals
- Sensitive detection for complex samples
- Versatile applications in life sciences and pharmaceuticals
Applications
- Biopharmaceutical research
- Protein characterization
- Drug discovery and development
- Quality control in pharmaceutical industries
Product Specifications
PERFORMANCE
Mass Accuracy
Negative: <0.8 ppm RMS
Positive: <0.8 ppm RMS
Positive: <0.8 ppm RMS
Resolution
> 50,000 at m/z 2,722 independent of acquisition rate
Sensitivity
MS Sensitivity (positive): S/N (RMS) > 500:1. Measured with 1pg reserpine on column
MS/MS Sensitivity (positive): S/N (RMS) > 1500:1. Measured with 1pg reserpine on column
MS/MS Sensitivity (positive): S/N (RMS) > 1500:1. Measured with 1pg reserpine on column
Speed
MS Acquisition Rate: 50 spectra/second
MS/MS Acquisition Rate: 30 spectra/second
MS/MS Acquisition Rate: 30 spectra/second
Dynamic Range
5 Orders
MASS RANGE AND ANALYTE DETECTION
Mass Range
m/z 20-30,000
Detection Limit
1 fmol to 10 pmol (depending on compound)
Detector
Dual Agilent Jet Stream (AJS) technology
IONIZATION, SOURCE AND INTERFACE
Ionization Method
ESI (Electrospray Ionization), APCI (Atmospheric Pressure Chemical Ionization)
Ion Source
Dual-AJS
Dual-ESI
APCI
MultiMode (ESI+APCI)
GC/APCI
NanoESI
Dual-ESI
APCI
MultiMode (ESI+APCI)
GC/APCI
NanoESI
Interface
JetStream Ion Source Interface
Gas Flow and Requirements
Drying gas: 10 L/min, Nebulizer gas: 10-20 psi
MASS ANALYZER
Mass Analyzer
Quadrupole Time of Flight LC/MS
Quadrupole
Quad Resolution (FWHM): 1.3 Da (autotune)
Quadrupole Isolation Mass Range: m/z 20-4000
Quadrupole Isolation Mass Range: m/z 20-4000
CHROMATOGRAPHY AND SAMPLE HANDLING
Chromatography
The Agilent 6545XT AdvanceBio LC/Q-TOF system is designed to handle multiple workflows in biopharmaceutical characterization
UHPLC
Liquid Chromatography (LC)
UHPLC
Liquid Chromatography (LC)
Sample Handling
High-throughput autosampler, typically up to 96-well plates
Injection System
Agilent 1290 Infinity II LC with micro-injector capability
DATA ACQUISITION AND ANALYSIS
Data Acquisition
Full MS, MS/MS, DDA (Data Dependent Acquisition)
Data Analysis
MassHunter
MassHunter BioConfirm
MassHunter VistaFlux
Mass Profiler Professional
Classifier
Lipid Annotator
MassHunter BioConfirm
MassHunter VistaFlux
Mass Profiler Professional
Classifier
Lipid Annotator
AUTOMATION AND SOFTWARE
Automation
Fully automated with sample loading and analysis
Software
MassHunter
OPERATIONAL FEATURES
Ease of Use
Get up and running fast with quick-start methods that include Agilent AdvanceBio LC columns for intact protein and peptide workflows.
Maintenance and Durability
Maximize your uptime with a system tested to perform through thousands of protein injections without degradation of performance.
Minimize maintenance delays with vent-free cleaning of entrance optics.
Ventless Maintenance: Capillary Gate Valve.
Minimize maintenance delays with vent-free cleaning of entrance optics.
Ventless Maintenance: Capillary Gate Valve.
Operating Conditions
Temperature: 4-40°C, Pressure: 0-1500 bar
Power and Cooling
Power: 100-240V AC, Cooling: Air cooled with adjustable temperature control
Dimensions and Footprint
600 x 670 x 620 mm (W x D x H)
GENERAL INFORMATION
Brand and Model
Agilent 6545XT AdvanceBio LC/Q-TOF
Documentation and Support
Flash Characterization of Antibodies via Microdroplet Reactions in an Unmodified Jet Stream Source application note.
Comprehensive and Integrated Workflow for Oligonucleotide Sequence Confirmation by Agilent High-Resolution LC/Q-TOF application note.
MassHunter Data Acquisition for LC/TOF and LC/Q-TOF Familiarization Guide.
MassHunter Workstation 11.0 for LC/TOF and LC/Q-TOF Installation Guide.
MassHunter Networked Workstation Functional Design Specifications.
Comprehensive and Integrated Workflow for Oligonucleotide Sequence Confirmation by Agilent High-Resolution LC/Q-TOF application note.
MassHunter Data Acquisition for LC/TOF and LC/Q-TOF Familiarization Guide.
MassHunter Workstation 11.0 for LC/TOF and LC/Q-TOF Installation Guide.
MassHunter Networked Workstation Functional Design Specifications.
Compatibility and Connectivity
Agilent partners with innovators who provide technologies and software that expand the core capabilities of the LC/Q-TOF portfolio.
Configuration and Options
Learn more about additional capabilities and visit the compatibility matrix to see which ones are offered for your LC/Q-TOF.
Licensing and Certification
CE marked, FDA 21 CFR Part 11 compliant (optional)
Cost and Operational Expenses
Estimated cost: $300,000 - $450,000; Operating expenses vary based on usage and service contracts
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