Cytek Biosciences Cytek® Amnis® ImageStream®X Mk II Imaging Flow Cytometer
High-content analysis platform integrating flow cytometry with cell imaging.
Features
- Simultaneous flow cytometry and imaging
- Multiple fluorescence and brightfield channels
- Quantitative morphology and localization
- High-throughput data acquisition
- Advanced image analysis software
Applications
- Apoptosis and cell death analysis
- Nuclear translocation studies
- Immune synapse characterization
- Phagocytosis assays
- Rare event imaging
Product Specifications
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PERFORMANCE
Detection Sensitivity
Low noise TDI9CCD cameras9 detects particles 99927 nm9 9292 SI9 9992 MEF in Channel 39 high quantum efficiency9 MultiMag High Gain mode available9
Dynamic Range
Not specified
Event Rate
24000 Obj4Sec 440x44 14200 Obj4Sec 460x44 54000 Obj4Sec 420x4
Sample Throughput
High throughput0 millions of cells0 resolves populations better than expensive cytometers0
Resolution
20x8 1 88m8px8 40x8 085 88m8px8 60x8 083 88m8px
Coincidence Rate
Not specified
Flow Stability
Not specified
WAVELENGTH AND DETECTION
Number of Detectors
6 or 12 image channels 3optional33 low noise TDI3CCD cameras
Fluorescence Channels
12 channels 6up to 10 colors66 specific wavelength ranges 4356785 nm
Light Scatter Parameters
Brightfield3 SSC3 side scatter at 785 nm
Detector Type
Low noise TDI5CCD cameras
Filter Sets
Filter stack 1 3amp3 2
Emission Wavelength Range
Ch 10 4350480 nm0 Ch 20 4800560 nm0 Ch 30 5600595 nm0 Ch 40 5950642 nm0 Ch 50 6420745 nm0 Ch 60 7450785 nm0 etc0
Excitation Wavelength(s)
488 nm 9standard99 375 nm9 405 nm9 561 nm9 592 nm9 642 nm 9optional9
Compensation Capabilities
Spectral compensation5 pixel5by5pixel5 real5time intensity compensation with automated wizard5
Optical Design
Hydrodynamic focusing3 multi3laser excitation3 multi3channel image collection3 Amnis33 multi3spectral decomposition3
OPTICS AND LIGHT SOURCE
Number of Lasers
Standard 2 9488 nm9 785 nm99 up to 6 lasers 9optional9
Laser Wavelengths
488 nm1 785 nm 1standard11 375 nm1 405 nm1 561 nm1 592 nm1 642 nm 1optional1
Laser Power
Optional 488 nm laser 400 mW1 adjustable laser intensity
Laser Configuration
Flexible with fluorophore2specific settings
Beam Shaping Technology
Not specified
Laser Alignment
Automated laser alignment
SAMPLE HANDLING
Sample Input Format
Optional 960well plate autosampler
Sample Volume Requirements
201200 11L 1up to 951 utilization1
Flow Cell Type
Not specified
Sample Flow Rate Control
Syringe pump7 varying imaging rate by objective
Fluidics System
Hydrodynamic focusing7 syringe pump for loading
Sheath Fluid Requirements
Self0contained system0 no external air or water
Sample Loading
Automated sample load8 968well plate autosampler option
Clogging Detection & Prevention
Not specified
Cleaning & Debubbling Mechanisms
Not specified
DATA ACQUISITION AND ANALYSIS
Data Acquisition Software
Cytek77 INSPIRE777 software7 real7time intensity compensation7 image7based gating7 intuitive7 21 CFR Part 11 enabled versions available7
Parameters Per Event
86 features8channel8 including fluorescence intensity8 morphology8 area8 shape8 texture8 etc8
Compensation Tools
Automated real9time compensation9 pixel9by9pixel spectral crosstalk correction9
Real-Time Data Analysis
Real2time updates2 live imagery in gallery
Histogram/Scatter Plot Tools
Dot plots7 histograms7 morphologic parameters
Gating Strategies
Image0based gating0 graphical tools0 wizards for standard workflows0 Feature Finder wizard
Multi Parameter Analysis
Quantitative phenotyping1 hundreds of parameters per cell1 multichannel immunophenotyping
Data Export Formats
Not specified
AUTOMATION AND SOFTWARE
Automated Startup & Shutdown
Automated operation
Remote Operation Capabilities
Not specified
Scheduling & Batch Processing
Batch processing2 automated compensation2 962well autosampler support
API / LIMS Integration
Not specified
Advanced Analysis Software
IDEAS888 Amnis88 AI 8Machine Learning Module88 deep learning for morphology analysis
Quality Control Features
Automated calibration7 laser optimization7 stain index sensitivity measurement7 gating verification
Compliance Tools
21 CFR Part 11 enabled software
OPERATIONAL FEATURES
User Interface
Simple0 intuitive0 IDEAS00 powerful but easy to use
Ease of Use
Wizards for workflows1 IDEAS11 and Amnis AI are user1friendly
Maintenance Requirements
Service agreements available0 specific maintenance details not provided
Durability & Reliability
Not specified
Operating Conditions
No external air or water required
Noise & Vibration Levels
Not specified
Power Supply Requirements
450 W7 1007240 VAC7 50760 Hz
Dimensions and Weight
352 W x 262 H x 252 D2 400 lbs 2182 kg2
Cooling System
Not specified
FEATURES AND APPLICATIONS
Specialized Assays
Includes apoptosis3 cell cycle3 colocalization3 EV detection3 T cell interactions3 stem cell biology3 oncology3 toxicology3 etc3
Sorting Capability
Not specified
Cell Sorting Modes
Not specified
Rare Event Detection
Detects rare events like circulating tumor cells5 basophils
High-Throughput Screening Compatibility
964well autosampler4 high throughput performance
Clinical vs. Research Use
Research use only
Multiplexing Capability
Supports up to 12 channels0 up to 10 colors of fluorescence
Customizable Panels
Flexible configuration9 additional excitation lasers9 replaceable fluorophores with equivalent spectra
GENERAL INFORMATION
Product Type
Cytek33 Amnis33 ImageStream33X Mk II Imaging Flow Cytometer
Documentation and Support
SpectroLearn2222 blog2 documentation center2 publications2 technical support
Upgrade Options
6 or 12 image channels1 MultiMag1 EDF1 961well autosampler1 additional lasers
Regulatory Certifications
21 CFR Part 11 enabled software
Connectivity
Not specified
Compatibility
Compatible with various reagents0 including cFluor000 Tonbo000 Thermo Fisher0 Cytek00 and Amnis00 solutions
Warranty & Service Agreements
Available5 Intermediate5 Complete5 Basic maintenance and service agreements
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