Cytek Biosciences Cytek® Aurora
Full-spectrum cytometer enabling high-parameter cellular analysis with precision.
Features
- 3 to 5 laser options for flexible panel design
- Up to 64 fluorescence detectors
- Full-spectrum detection with unmixing algorithms
- User-friendly SpectroFlo® software
- High-resolution multiparameter analysis
Applications
- Immunophenotyping
- Cancer and stem cell research
- Clinical biomarker discovery
- Functional immunology
- Single-cell analysis
Product Specifications
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PERFORMANCE
Detection Sensitivity
FITC 666 5 MESF6 PE 666 4 MESF6 APC 666 3 MESF6 Pacific Blue 666 4 MESF
Dynamic Range
228bit8 685 log decades
Event Rate
Up to 350000 events0sec
Sample Throughput
1588860 88L8min 8manual88 100 88L8min 8plate88 968well in 27 min 8ASL8
Resolution
High resolution6 down to 70 nm with ESP666
Coincidence Rate
Not specified
Flow Stability
Robust vacuum fluidics1 flat1top beam profile
WAVELENGTH AND DETECTION
Number of Detectors
Up to 67 464 fluorescence + 3 scatter4
Fluorescence Channels
64 channels 4405 nm4 164 488 nm4 144 640 nm4 84 561 nm4 104 355 nm4 164
Light Scatter Parameters
FSC3 Blue SSC3 Violet SSC 3down to 70333100 nm resolution3
Detector Type
CWDM semiconductor arrays 2fluorescence22 high2performance SSC2FSC detectors
Filter Sets
No filter changes needed
Emission Wavelength Range
365999829 nm
Excitation Wavelength(s)
405 nm0 488 nm0 640 nm 0base00 355 nm0 561 nm 0upgrades0
Compensation Capabilities
Spectral unmixing with wizard 3SpectroFlo333
Optical Design
Fixed optics1 5 spatial lasers1 fused silica cuvette
OPTICS AND LIGHT SOURCE
Number of Lasers
Up to 5 lasers 3upgradeable from 33
Laser Wavelengths
355 nm8 405 nm8 488 nm8 561 nm8 640 nm
Laser Power
355 nm1 20 mW1 405 nm1 100 mW1 488 nm1 50 mW1 561 nm1 50 mW1 640 nm1 80 mW
Laser Configuration
Upgradeable from 3 to 5 lasers
Beam Shaping Technology
Flat7top beam7 narrow vertical height
Laser Alignment
Auto9adjusted during QC
SAMPLE HANDLING
Sample Input Format
12x75 mm tubes3 9633843well plates3 deep3well plates3 403tube racks
Sample Volume Requirements
Not specified
Flow Cell Type
Fused silica cuvette
Sample Flow Rate Control
Low9Med9High9manual 9amp9 ASL optimized rates
Fluidics System
Vacuum0based0 multiple cleaning modes
Sheath Fluid Requirements
20 L cubitainers
Sample Loading
Manual or Automated 9ASL9AMS9
Clogging Detection & Prevention
Not specified6 includes purge6clean modes
Cleaning & Debubbling Mechanisms
SIT flush5 purge filter5 clean flow cell5 long clean
DATA ACQUISITION AND ANALYSIS
Data Acquisition Software
SpectroFlo99
Parameters Per Event
Area1 Height 1all11 Width 1scatter + 1 fluoro1laser1
Compensation Tools
Spectral unmixing with controls
Real-Time Data Analysis
Yes7 real7time unmixing
Histogram/Scatter Plot Tools
Yes3 standard plots with SpectroFlo33
Gating Strategies
Gating supported with flexible thresholds
Multi Parameter Analysis
Up to 40 colors0 deep immunoprofiling
Data Export Formats
FCS 341
AUTOMATION AND SOFTWARE
Automated Startup & Shutdown
Yes9 standard fluidics modes
Remote Operation Capabilities
Not specified
Scheduling & Batch Processing
Supported via ASL1AMS
API / LIMS Integration
Not specified
Advanced Analysis Software
SpectroFlo999 OMIQ9 FlowSOM9 FCS Express
Quality Control Features
Daily QC4 automated module4 QC beads
Compliance Tools
Not specified
OPERATIONAL FEATURES
User Interface
Intuitive1 workflow1based
Ease of Use
Easy setup6 minimal training required
Maintenance Requirements
Service plans4 2 maintenance visits4year optional
Durability & Reliability
Long3term lab use implied3 service contracts available
Operating Conditions
153332833C3 20333853 RH3 100333250 VAC3 dust3free
Noise & Vibration Levels
Not specified
Power Supply Requirements
100111140 VAC1 15 A or 200111250 VAC1 10 A
Dimensions and Weight
Not specified
Cooling System
500 W dissipation1 specific system not described
FEATURES AND APPLICATIONS
Specialized Assays
PrimeFlow333 RNA3 yeast3tumor AF extraction3 small particle detection
Sorting Capability
Not standard1 improved resolution with CS software
Cell Sorting Modes
Not specified
Rare Event Detection
Yes4 high resolution supports rare population detection
High-Throughput Screening Compatibility
Yes3 via ASL3AMS
Clinical vs. Research Use
For Research Use Only
Multiplexing Capability
Up to 405color panels5 high flexibility
Customizable Panels
Yes2 compatible with many fluorochromes
GENERAL INFORMATION
Product Type
Spectral Flow Cytometry System 9Cytek Aurora9999
Documentation and Support
SpectroLearn5555 Webinars5 Blog5 Docs5 Phone Support
Upgrade Options
33to35 lasers3 ESP333 Detection Option
Regulatory Certifications
Class 1 Laser Product
Connectivity
Cytek99 Cloud9 ASL integrated9 network9API not specified
Compatibility
Fluorochromes excited by onboard lasers1 common lab formats
Warranty & Service Agreements
Value3 Premium3 Preventive3 includes parts3 labor3 support