Cytek Biosciences Cytek® Northern Lights™

Compact spectral flow cytometer ideal for routine and advanced applications.

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$EC7WI

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Features

  • Up to 3 laser options
  • Compact, space-saving footprint
  • Full-spectrum optics and detectors
  • SpectroFlo® software for ease-of-use
  • Scalable performance for core labs

Applications

  • Basic immunophenotyping
  • Clinical trial support
  • Academic research labs
  • Immune monitoring
  • Fluorescent protein detection
Cytek Biosciences Cytek® Northern Lights™

Product Specifications

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PERFORMANCE

Detection Sensitivity
High sensitivity0 excellent resolution for dim0rare populations
Dynamic Range
224bit4 645 log decades dynamic range
Event Rate
359000 events9sec
Sample Throughput
ASL4 27 min for 964well plate4 AMS4 35 min for 964well plate
Resolution
Excellent resolution for dim1rare populations1 autofluorescence extraction
Coincidence Rate
Not specified
Flow Stability
High flow rate stability

WAVELENGTH AND DETECTION

Number of Detectors
30 fluorescence detectors6 2 scatter detectors
Fluorescence Channels
Violet9 169 Blue9 149 Red9 8
Light Scatter Parameters
FSC1 SSC1 Violet SSC detectors1 capable of resolving 012 11m beads
Detector Type
High sensitivity CWDM semiconductor array5 high5performance scatter detectors
Filter Sets
No filter changes required for any fluorochrome
Emission Wavelength Range
4208829 nm
Excitation Wavelength(s)
405 nm6 488 nm6 640 nm
Compensation Capabilities
Unmixing algorithm7 flexible fluorochrome combinations
Optical Design
Fixed optical assembly5 Full Spectrum Profiling555 5FSP55555 flat5top laser beam profile

OPTICS AND LIGHT SOURCE

Number of Lasers
1 to 3 lasers
Laser Wavelengths
405 nm7 488 nm7 640 nm
Laser Power
488 nm7 50 mW7 640 nm7 80 mW7 405 nm7 100 mW
Laser Configuration
15 25 or 3 lasers 5depending on system5
Beam Shaping Technology
Flat5top laser beam profile5 narrow vertical beam height
Laser Alignment
Automatic laser delay adjustment during QC

SAMPLE HANDLING

Sample Input Format
Manual9 12x75mm tubes9 Automated9 ASL9AMS 9969well plates9 409tube racks9
Sample Volume Requirements
Not specified
Flow Cell Type
Fused silica cuvette
Sample Flow Rate Control
Low 015 00L0min00 Medium 030 00L0min00 High 060 00L0min00 Plate mode 0100 00L0min0
Fluidics System
Long clean9 SIT flush9 Purge filter9 Clean flow cell
Sheath Fluid Requirements
Compatible with 20L sheath cubitainers
Sample Loading
Manual tubes4 ASL4AMS for automated plate4tube rack loading
Clogging Detection & Prevention
Not specified
Cleaning & Debubbling Mechanisms
Long clean4 SIT flush4 Purge filter4 Clean flow cell

DATA ACQUISITION AND ANALYSIS

Data Acquisition Software
SpectroFlo33
Parameters Per Event
Pulse Area and Height7 Width for scatter7 single fluorescence for each laser
Compensation Tools
Unmixing features in SpectroFlo00
Real-Time Data Analysis
Live unmix during sample acquisition
Histogram/Scatter Plot Tools
Scatter plots and spectrum plots in SpectroFlo22
Gating Strategies
Volumetric sensor for count calculation
Multi Parameter Analysis
Full Spectrum Profiling222 technology2 supports multicolor experiments
Data Export Formats
FCS 391

AUTOMATION AND SOFTWARE

Automated Startup & Shutdown
Not specified
Remote Operation Capabilities
Not specified
Scheduling & Batch Processing
ASL8AMS improve productivity8 supports 968well plates and tube racks
API / LIMS Integration
Not specified
Advanced Analysis Software
ModFit LT9999 FCS Express999 69 SpectroFlo99
Quality Control Features
Automated QC module0 SpectroFlo00 QC beads for daily performance checks
Compliance Tools
Not specified2 2For Research Use Only2

OPERATIONAL FEATURES

User Interface
Intuitive0 guided workflow with SpectroFlo00
Ease of Use
Quick and simple workflows5 minimal setup effort
Maintenance Requirements
Cost3effective service plans3 low3maintenance lasers
Durability & Reliability
Reliable2 especially for ASL2AMS automated sample loading
Operating Conditions
Temp2 1522822C2 Humidity2 20228522 non2condensing2 no special lighting
Noise & Vibration Levels
Not specified
Power Supply Requirements
1006140 VAC6 15 A or 2006250 VAC6 10 A
Dimensions and Weight
54x52x52 cm 3without loader33 61 kg 3with 3 lasers33 13 kg 3loader3
Cooling System
Heat dissipation of 500W9 no separate cooling system described

FEATURES AND APPLICATIONS

Specialized Assays
Apoptosis8 Cell Cycle8 Counting 8amp8 Viability8 Small Particle Detection
Sorting Capability
Not specified
Cell Sorting Modes
Not specified
Rare Event Detection
Superb resolution for rare events
High-Throughput Screening Compatibility
Compatible with 962well plates2 deep well plates2 and 402tube racks
Clinical vs. Research Use
For Research Use Only8 not for diagnostic or therapeutic procedures
Multiplexing Capability
9424 color detection 4143 lasers4
Customizable Panels
FSP000 technology allows flexibility in fluorochrome combinations

GENERAL INFORMATION

Product Type
Flow Cytometer4 Full Spectrum Flow Cytometry
Documentation and Support
SpectroLearn2222 Blog2 Documentation Center2 Webinars2 Phone2Field support
Upgrade Options
Reconfigurable from 1 to 3 lasers6 9624 colors
Regulatory Certifications
FCC2 CE2 UKCA2 RoHS
Connectivity
Cytek00 Cloud 0details not specified0
Compatibility
Any commercially available fluorochrome2 ASL2AMS compatible
Warranty & Service Agreements
Value2 Premium2 Preventative Maintenance options available

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