Thermofisher Orbitrap Eclipse™ Tribrid™ Mass Spectrometer
Orbitrap Eclipse™ Tribrid™ MS: Advanced Ion Management, PTCR, and HMRⁿ mode for high-throughput proteomics and complex analyses.
Features
- Advanced Ion Management Technology (AIM+)
- Real-Time Search for enhanced TMT analysis
- High Mass Range MS^{n} (HMR^{n}) mode
- Proton Transfer Charge Reduction (PTCR)
- Industry-leading precursor selectivity
- Comprehensive experimental flexibility
Applications
- Full-proteome quantification
- Protein characterization
- Small molecule-based pharmaceuticals analysis
- Deciphering native protein complexes and higher-order structures
Product Specifications
PERFORMANCE
Mass Accuracy
<3 ppm RMS using external calibration, <1 ppm RMS using internal calibration, <1 ppm RMS drift over 24 hours, measured with Flex Mix
Resolution
Up to 500,000 at m/z 200
Optional: Up to 1,000,000 at m/z 200
Optional: Up to 1,000,000 at m/z 200
Sensitivity
- 2 µL of 50 fg/µL reserpine (100 fg total) at 500 µL/min
- Minimum S/N ratio: 200:1
- Transition: m/z 609 → m/z 397, m/z 448
- Unit resolution, full scan MS/MS mode (m/z 165)
Speed
Orbitrap: Up to 40 Hz
Linear Ion Trap: Up to 45 Hz
Linear Ion Trap: Up to 45 Hz
Dynamic Range
>5000 within a single Orbitrap mass spectrum
MASS RANGE AND ANALYTE DETECTION
Mass Range
Standard: 50 to 2000 m/z
Mid: 200 to 6000 m/z
Optional (HMRn): 500 to 8000 m/z
Mid: 200 to 6000 m/z
Optional (HMRn): 500 to 8000 m/z
Detection Limit
Low fmol (femtomolar) range for qualitative and quantitative analysis
Detector
Large surface area ion trap detector
IONIZATION, SOURCE AND INTERFACE
Ionization Method
Heated electrospray ionization (HESI)
Ion Source
Thermo Scientific™ Ion Max NG™ ion source
Interface
API interface consists of a High-Capacity Transfer Tube (HCTT) and an Electrodynamic Ion Funnel (EDIF)
Gas Flow and Requirements
- High-purity Nitrogen (99.5%, 15 L/min) for API source and IRM
- Ultra-high purity Helium (99.999%) with <1 ppm water, oxygen, and total hydrocarbons
MASS ANALYZER
Mass Analyzer
Orbitrap1311
Tribrid architecture includes a quadrupole mass filter, dual-pressure linear ion trap and Orbitrap mass analyzers
Tribrid architecture includes a quadrupole mass filter, dual-pressure linear ion trap and Orbitrap mass analyzers
Quadrupole
QR5 segmented quadrupole mass filter with hyperbolic surfaces
CHROMATOGRAPHY AND SAMPLE HANDLING
Chromatography
LC-MS
Sample Handling
Automatic sample introduction, compatible with liquid and solid samples
Injection System
Standard and enhanced ionization sources, including ESI, APCI, and more
DATA ACQUISITION AND ANALYSIS
Data Acquisition
HRAM
Data Analysis
Real-Time Search
AUTOMATION AND SOFTWARE
Automation
Fully automated analysis, including sample preparation, injection, and data analysis
Software
Thermo Scientific™ Xcalibur™ software15, Orbitrap Eclipse MS instrument control software
OPERATIONAL FEATURES
Ease of Use
Intuitive, flexible drag-and-drop instrument method editor simplifies method development and creation of unique analytical workflows16
Extensive method template library
Extensive method template library
Maintenance and Durability
Enhanced Vacuum Technology reduces the pressure in the ultra-high vacuum region to <10-10 Torr17
Stainless steel and aluminum high-vacuum analyzer chambers
Stainless steel and aluminum high-vacuum analyzer chambers
Operating Conditions
16–26°C (59–78°F) and relative humidity must be 50–80% with no condensation
Power and Cooling
230 Vac ±10% single phase, 50/60 Hz, with ground, 3x outlets at 15 A each
System averages 2800 W (10,000 Btu/hr) output
System averages 2800 W (10,000 Btu/hr) output
Dimensions and Footprint
1270 × 767 × 703 mm (50 × 30.2 × 27.7 in)
GENERAL INFORMATION
Brand and Model
Thermo Scientific™, Orbitrap Eclipse™ Tribrid™ Mass Spectrometer
Documentation and Support
Comprehensive documentation
24/7 technical support, software updates
24/7 technical support, software updates
Compatibility and Connectivity
Compatible with various chromatography systems (LC, GC) and network integration
Configuration and Options
Modular design with multiple ionization sources, collision cells, and detectors
Licensing and Certification
CE certified
RoHS compliant
ISO 9001 certified
RoHS compliant
ISO 9001 certified
Cost and Operational Expenses
High initial investment
Moderate operational expenses for consumables, maintenance contracts optional
Moderate operational expenses for consumables, maintenance contracts optional
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