Bruker PepSep Columns
PepSep Columns offer high separation quality, low dead volume, and reproducibility for optimal LC-MS proteomics analysis.
Features
- High-resolution separation for LC-MS workflows
- Low dead volume ensuring precision and efficiency
- Optimized for proteomics and metabolomics studies
- Exceptional reproducibility and reliability for consistent results
Applications
- Proteomics research
- Metabolomics studies
- Biomarker discovery
- Pharmaceutical and clinical research
- Life science analytical workflows
Product Specifications
PERFORMANCE
Mass Accuracy
±0.1 Da
Resolution
High resolution
Sensitivity
Superior sensitivity
Ultra-high sensitivity
High 4D-sensitivity
Mega-sensitivity
Ultra-high sensitivity
High 4D-sensitivity
Mega-sensitivity
Speed
High throughput
PASEF® speeds
Fast analysis times
Short gradients
PASEF® speeds
Fast analysis times
Short gradients
Dynamic Range
High dynamic range
MASS RANGE AND ANALYTE DETECTION
Mass Range
50–10,000 m/z
Detection Limit
Sub-ng to picomolar levels
Detector
UV/Vis Detector or MS Detection
IONIZATION, SOURCE AND INTERFACE
Ionization Method
ESI (Electrospray Ionization)
Ion Source
CaptiveSpray Sources
Interface
nanoElute®
Gas Flow and Requirements
0.1–0.3 L/min (dry nitrogen)
MASS ANALYZER
Mass Analyzer
timsTOF series
timsTOF Pro
timsTOF HT
timsTOF Pro
timsTOF HT
Quadrupole
N/A (Not applicable for columns)
CHROMATOGRAPHY AND SAMPLE HANDLING
Chromatography
High-performance liquid chromatography (HPLC)
Reversed phase
Reversed phase
Sample Handling
Automatic sample injectors
Injection System
Manual or automatic liquid injection
DATA ACQUISITION AND ANALYSIS
Data Acquisition
MS
LC-MS
LC-MS/MS data systems
LC-MS
LC-MS/MS data systems
Data Analysis
Bruker ProteoScape™
DIA-NN
MSFragger
Spectronaut with directDIA+
DIA-NN
MSFragger
Spectronaut with directDIA+
AUTOMATION AND SOFTWARE
Automation
Compatible with automated systems
Software
Bruker ProteoScape™
OPERATIONAL FEATURES
Ease of Use
Simple twist on and twist off design
Plug and play ready-to-use emitters
Plug and play ready-to-use emitters
Maintenance and Durability
Outlasting leading columns up to 2x longer
Operating Conditions
25–40°C (typically room temperature)
Power and Cooling
Not applicable (Passive cooling)
Dimensions and Footprint
Various lengths, inner diameters, and particle sizes
GENERAL INFORMATION
Brand and Model
PepSep® Columns & Emitters
PepSep® ULTRA XL
PepSep® MAX HT
PepSep® ULTRA
PepSep® XTREME
PepSep® MAX
PepSep® ULTRA XL
PepSep® MAX HT
PepSep® ULTRA
PepSep® XTREME
PepSep® MAX
Documentation and Support
Available through Bruker
Compatibility and Connectivity
- timsTOF Series Instruments
- nanoElute® UHPLC
- timsTOF Pro
- 5 mm Thermo PepMap Trap
- CaptiveSpray Sources
Configuration and Options
Diverse portfolio of nanoflow columns
Licensing and Certification
For Research Use Only. Not for use in clinical diagnostic procedures.
Cost and Operational Expenses
Depends on usage and consumables