Sony Biotechnology Inc. FX500 Exchangeable Fluidics Cell Sorter

Cell sorter with unique exchangeable fluidics for contamination-free performance.

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$RW6Y34

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Features

  • Exchangeable fluidics system
  • Reduced contamination risk
  • Multiple laser support
  • User-focused interface
  • Reliable and repeatable results

Applications

  • Cell therapy manufacturing
  • Pathogen-sensitive workflows
  • Sterile cell sorting
  • GMP environments
  • Cell cloning

Product Specifications

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PERFORMANCE

Detection Sensitivity
FITC6 120 MESF6 PE6 110 MESF 6Spherotech beads6
Dynamic Range
85channel 205bit ADC5 1105100 MHz
Event Rate
1002000 eps
Sample Throughput
129000 eps 9 9gt9989 purity9 9gt9809 yield 9Normal Mode99 up to 309000 eps with reduced yield
Resolution
Scatter8 085 88m8 Fluorescence8 8882858 HPCV
Coincidence Rate
Yield decrease per Poisson statistics3 value not specified
Flow Stability
Auto drop drive adjustments for stability

WAVELENGTH AND DETECTION

Number of Detectors
8 total 86 fluorescence detectors8
Fluorescence Channels
6 2via 6 free2form PMTs2
Light Scatter Parameters
FSC3 BSC with filters
Detector Type
Free3form PMTs
Filter Sets
FSC5 488517F5 BSC5 488517B5 FL1555FL65 LPs5 639LP5 600LP5 etc5
Emission Wavelength Range
Defined by filter sets
Excitation Wavelength(s)
488 nm7 638 nm7 561 nm
Compensation Capabilities
Software wizards and compensation tube setup
Optical Design
Collinear optical system

OPTICS AND LIGHT SOURCE

Number of Lasers
3
Laser Wavelengths
488 nm0 638 nm0 561 nm
Laser Power
15 mW 5typical and max55 per laser
Laser Configuration
Collinear
Beam Shaping Technology
Not specified
Laser Alignment
Auto7align using Blu7Ray777 tech and AutoSetup beads

SAMPLE HANDLING

Sample Input Format
Single1 auto1loading tube
Sample Volume Requirements
0252 1252 52 15 mL tubes
Flow Cell Type
Microfluidics chip
Sample Flow Rate Control
Pressure2driven2 up to 1002000 eps
Fluidics System
Replaceable3 pump3free3 gamma3E3beam sterilized components
Sheath Fluid Requirements
Gamma irradiated line6 pressurized stainless tank
Sample Loading
Auto8sealed lines during chip load
Clogging Detection & Prevention
Software9enabled detection and chip declogging
Cleaning & Debubbling Mechanisms
Automated purge9prime cycle9 debubbling not specified

DATA ACQUISITION AND ANALYSIS

Data Acquisition Software
Guided workflows8 dot plots8 histograms
Parameters Per Event
8 detection9 Height9 Area9 Width
Compensation Tools
Software wizard1 setup for tubes
Real-Time Data Analysis
Live plots7 auto drop drive tuning
Histogram/Scatter Plot Tools
Yes
Gating Strategies
Yes
Multi Parameter Analysis
Yes
Data Export Formats
FCS 3707 371

AUTOMATION AND SOFTWARE

Automated Startup & Shutdown
Fully automated with diagnostics
Remote Operation Capabilities
Not specified
Scheduling & Batch Processing
Not specified
API / LIMS Integration
Not specified
Advanced Analysis Software
FX500 software or export to 3rd party tools
Quality Control Features
Auto alignment3 calibration3 linearity3 diagnostics
Compliance Tools
FCS 33033313 Windows 103 UL3 CE3 CSA3 optional BCC 3NSF493EN124693

OPERATIONAL FEATURES

User Interface
Ribbon layout4 LCD status screen
Ease of Use
High automation1 CoreFinder1111 wizards
Maintenance Requirements
Replaceable chip3lines3 auto cleaning
Durability & Reliability
Automated systems ensure consistency2 metrics not specified
Operating Conditions
1705000270500C0 20000800 RH
Noise & Vibration Levels
Not specified
Power Supply Requirements
100V5120V 50560 Hz5 500 W max5
Dimensions and Weight
5533553372 cm3 100 kg3 Fluidics Cart3 7135333435337035 cm3 37 kg
Cooling System
Electric3 533C for tubes3 3733C for samples

FEATURES AND APPLICATIONS

Specialized Assays
T cell sorting7 index sorting
Sorting Capability
25way tubes5 plates5 PCR tubes5 slides
Cell Sorting Modes
Normal8 Single8cell8 High8purity
Rare Event Detection
Not explicitly listed5 supports high purity sorting
High-Throughput Screening Compatibility
Yes8 968384 plates supported via Sort Deposition System
Clinical vs. Research Use
Research only
Multiplexing Capability
6 fluorescence channels0 flexible filters
Customizable Panels
Yes2 via filter changes

GENERAL INFORMATION

Product Type
Microfluidics chip9based cell sorter
Documentation and Support
Yes8 contact info listed in brochure
Upgrade Options
Sort Deposition System5 BCC3005 chip sizes 5705 1005 130 55m5
Regulatory Certifications
UL1 CE1 CSA1 Class 1 laser1 BCC1 NSF491 EN12469
Connectivity
Data export in FCS formats6 other network info not specified
Compatibility
FCS files5 Windows 105 multiple tube5plate formats
Warranty & Service Agreements
Not specified