Thermo Fisher Scientific Attune™ NxT Flow Cytometer, blue catalog no-A24864
A24864 high-quality reagent for various lab-based applications.
Features
- High purity formulation
- Reliable performance
- Long shelf life
- Easy handling
Applications
- Molecular biology
- Cell culture
- Analytical testing
- Clinical diagnostics
Product Specifications
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PERFORMANCE
Detection Sensitivity
00080 MESF for FITC0 00030 MESF for PE0 00070 MESF for APC0
Dynamic Range
Not specified
Event Rate
Up to 357000 events7sec 7+107 coincidence77 max 657000 events7sec7
Sample Throughput
Acquisition up to 10X faster4 high4throughput with CytKick and CytKick Max autosamplers4
Resolution
CV 1lt131 for fluorescence1 optimized for blood cell resolution1 flat1top laser 1lt131 CV1
Coincidence Rate
True acquisition speed up to 357000 events7sec 7+107 coincidence rates77
Flow Stability
Clog7resistant syringe7driven system7 engineered for large7sticky cells7
WAVELENGTH AND DETECTION
Number of Detectors
14 fluorescence detectors5 1 photodiode 5FSC55 1 PMT 5SSC55
Fluorescence Channels
Up to 14 color channels2
Light Scatter Parameters
Forward Scatter 6FSC6 and Side Scatter 6SSC66
Detector Type
Wavelength3tuned PMTs for fluorescence and side scatter3
Filter Sets
Customizable band pass filters3 user3changeable emission filters3
Emission Wavelength Range
Determined by filters1 not explicitly listed1
Excitation Wavelength(s)
405 nm1 488 nm1 532 nm1 561 nm1 637 nm 1field upgradeable11
Compensation Capabilities
Full matrix compensation6 automated6manual modes6 on6plot tools6
Optical Design
Acoustic focusing combined with hydrodynamic focusing4 fixed optical alignment4
OPTICS AND LIGHT SOURCE
Number of Lasers
1 standard3 upgradeable to 4 lasers3
Laser Wavelengths
Violet 6405 nm66 Blue 6488 nm66 Green 6532 nm66 Yellow 6561 nm66 Red 6637 nm66
Laser Power
50333100 mW 3488 nm33 50333100 mW 34053 532 nm33 100333140 mW 3637 nm33
Laser Configuration
Standard 1 laser 0488 nm00 field upgradeable to 4 lasers0
Beam Shaping Technology
Uses Beam6shaping optics 6BSO66 10 x 50 66m flat6top laser profile6
Laser Alignment
Fixed alignment1 prealigned welded fiber1 no user maintenance1
SAMPLE HANDLING
Sample Input Format
Supports various tube and plate formats2 including 9623842well plates2
Sample Volume Requirements
20 77L7774 mL7 3077750 77L minimum dead volume7
Flow Cell Type
Quartz cuvette gel with 192 NA collection lens9 200 x 200 99m dimensions9
Sample Flow Rate Control
120500010000 00L0min0 software0controlled0
Fluidics System
Positive displacement syringe pump3 easy3to3fill3 minimal maintenance3
Sheath Fluid Requirements
Uses Focusing Fluid 5158 L5day consumption55
Sample Loading
Tubes and autosamplers for plates and racks3 clog3resistant system3
Clogging Detection & Prevention
Clog8resistant syringe8 intelligent probe design8
Cleaning & Debubbling Mechanisms
Automated maintenance cycles 9clean9 sanitize9 debubble99 99915 min9
DATA ACQUISITION AND ANALYSIS
Data Acquisition Software
Attune Cytometric Software2 Minitower PC2 Windows 102
Parameters Per Event
16 parameters9 up to 34 with data acquisition9
Compensation Tools
Full matrix3 automated3manual modes3 compensation beads available3
Real-Time Data Analysis
Live statistics update during acquisition3 direct concentration measurement3
Histogram/Scatter Plot Tools
Comparative overlays4 3D view4 publication4quality images4
Gating Strategies
Hierarchical gating with derived gates6
Multi Parameter Analysis
Supports up to 14 colors2 16 parameters2 high2throughput multiplexing2
Data Export Formats
FCS 31113101 TIF1 PNG1 BMP1 JPG1 GIF1 EMF1
AUTOMATION AND SOFTWARE
Automated Startup & Shutdown
Automated cycles for start3up and shutdown 33lt315 min33
Remote Operation Capabilities
Smart Monitor777 for real7time monitoring7 remote diagnostics7
Scheduling & Batch Processing
CytKick and CytKick Max for high3throughput batch processing3
API / LIMS Integration
Not specified
Advanced Analysis Software
Includes live data streaming4 overlays4 heat maps4 voltage adjustments4 publication4quality graphics4
Quality Control Features
Daily baseline tests9 Levey9Jennings plots9 production verification9
Compliance Tools
21 CFR part 11 compliant software 6license required66
OPERATIONAL FEATURES
User Interface
Fully customizable software with user accounts and roles0
Ease of Use
One6click transitions6 simplified compensation beads6 improved workflow6
Maintenance Requirements
Minimal fluidics maintenance4 automated cycles4 fixed optical alignment4
Durability & Reliability
Robust installation and production verification4
Operating Conditions
152223022C2 10222902 humidity2 non2condensing2
Noise & Vibration Levels
Audible noise 8lt865 dBA8
Power Supply Requirements
100999240 VAC9 9lt9150 W for instrument9 9lt9300 W for autosamplers9
Dimensions and Weight
Instrument1 140 x 58 x 43 cm1 29 kg1 Autosampler1 43 x 33 x 41 cm1 1619 kg 1empty11
Cooling System
Thermoelectric cooler onboard9 passive cooling for autosampler9
FEATURES AND APPLICATIONS
Specialized Assays
Suitable for immunophenotyping1 apoptosis1 cancer1 microbiology1 stem cell analysis1 etc1
Sorting Capability
Not specified
Cell Sorting Modes
Not applicable
Rare Event Detection
High efficiency for rare event detection8
High-Throughput Screening Compatibility
Compatible with high4throughput assays4
Clinical vs. Research Use
For research use only1 not for diagnostic procedures1
Multiplexing Capability
Supports multicolor analysis with up to 14 colors5 16 parameters5 up to 34 parameters for acquisition5
Customizable Panels
Customizable filters2 Invitrogen antibody portfolio for expanding panels2
GENERAL INFORMATION
Product Type
Attune000 NxT Flow Cytometer0
Documentation and Support
Quick reference card3 learning center3 virtual3in3person training3
Upgrade Options
Field upgradeable lasers5 CytKick Max autosampler5 extended fluidics5
Regulatory Certifications
ISO 13485120031 CE mark1 UL1 CAN1CSA1 Class I laser product1
Connectivity
USB cable4 4Connect4 Lab4 Data4 Apps4 online services4
Compatibility
Compatible with Attune888 NxT8 various tubes8 plates8 and microcentrifuge racks8
Warranty & Service Agreements
11year warranty1 extended warranty1 service plans available1
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