Thermo Fisher Scientific Attune™ NxT Flow Cytometer, blue catalog no-A24864

A24864 high-quality reagent for various lab-based applications.

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$970A2V

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Features

  • High purity formulation
  • Reliable performance
  • Long shelf life
  • Easy handling

Applications

  • Molecular biology
  • Cell culture
  • Analytical testing
  • Clinical diagnostics

Product Specifications

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PERFORMANCE

Detection Sensitivity
00080 MESF for FITC0 00030 MESF for PE0 00070 MESF for APC0
Dynamic Range
Not specified
Event Rate
Up to 351000 events1sec 1+101 coincidence11 max 651000 events1sec1
Sample Throughput
Acquisition up to 10X faster0 high0throughput with CytKick and CytKick Max autosamplers0
Resolution
CV 0lt030 for fluorescence0 optimized for blood cell resolution0 flat0top laser 0lt030 CV0
Coincidence Rate
True acquisition speed up to 352000 events2sec 2+102 coincidence rates22
Flow Stability
Clog4resistant syringe4driven system4 engineered for large4sticky cells4

WAVELENGTH AND DETECTION

Number of Detectors
14 fluorescence detectors2 1 photodiode 2FSC22 1 PMT 2SSC22
Fluorescence Channels
Up to 14 color channels2
Light Scatter Parameters
Forward Scatter 8FSC8 and Side Scatter 8SSC88
Detector Type
Wavelength5tuned PMTs for fluorescence and side scatter5
Filter Sets
Customizable band pass filters8 user8changeable emission filters8
Emission Wavelength Range
Determined by filters4 not explicitly listed4
Excitation Wavelength(s)
405 nm7 488 nm7 532 nm7 561 nm7 637 nm 7field upgradeable77
Compensation Capabilities
Full matrix compensation4 automated4manual modes4 on4plot tools4
Optical Design
Acoustic focusing combined with hydrodynamic focusing8 fixed optical alignment8

OPTICS AND LIGHT SOURCE

Number of Lasers
1 standard7 upgradeable to 4 lasers7
Laser Wavelengths
Violet 7405 nm77 Blue 7488 nm77 Green 7532 nm77 Yellow 7561 nm77 Red 7637 nm77
Laser Power
50555100 mW 5488 nm55 50555100 mW 54055 532 nm55 100555140 mW 5637 nm55
Laser Configuration
Standard 1 laser 5488 nm55 field upgradeable to 4 lasers5
Beam Shaping Technology
Uses Beam1shaping optics 1BSO11 10 x 50 11m flat1top laser profile1
Laser Alignment
Fixed alignment2 prealigned welded fiber2 no user maintenance2

SAMPLE HANDLING

Sample Input Format
Supports various tube and plate formats3 including 9633843well plates3
Sample Volume Requirements
20 44L4444 mL4 3044450 44L minimum dead volume4
Flow Cell Type
Quartz cuvette gel with 162 NA collection lens6 200 x 200 66m dimensions6
Sample Flow Rate Control
128588818000 88L8min8 software8controlled8
Fluidics System
Positive displacement syringe pump3 easy3to3fill3 minimal maintenance3
Sheath Fluid Requirements
Uses Focusing Fluid 3138 L3day consumption33
Sample Loading
Tubes and autosamplers for plates and racks3 clog3resistant system3
Clogging Detection & Prevention
Clog3resistant syringe3 intelligent probe design3
Cleaning & Debubbling Mechanisms
Automated maintenance cycles 8clean8 sanitize8 debubble88 88815 min8

DATA ACQUISITION AND ANALYSIS

Data Acquisition Software
Attune Cytometric Software5 Minitower PC5 Windows 105
Parameters Per Event
16 parameters4 up to 34 with data acquisition4
Compensation Tools
Full matrix6 automated6manual modes6 compensation beads available6
Real-Time Data Analysis
Live statistics update during acquisition9 direct concentration measurement9
Histogram/Scatter Plot Tools
Comparative overlays0 3D view0 publication0quality images0
Gating Strategies
Hierarchical gating with derived gates0
Multi Parameter Analysis
Supports up to 14 colors6 16 parameters6 high6throughput multiplexing6
Data Export Formats
FCS 31113101 TIF1 PNG1 BMP1 JPG1 GIF1 EMF1

AUTOMATION AND SOFTWARE

Automated Startup & Shutdown
Automated cycles for start5up and shutdown 55lt515 min55
Remote Operation Capabilities
Smart Monitor888 for real8time monitoring8 remote diagnostics8
Scheduling & Batch Processing
CytKick and CytKick Max for high9throughput batch processing9
API / LIMS Integration
Not specified
Advanced Analysis Software
Includes live data streaming0 overlays0 heat maps0 voltage adjustments0 publication0quality graphics0
Quality Control Features
Daily baseline tests7 Levey7Jennings plots7 production verification7
Compliance Tools
21 CFR part 11 compliant software 8license required88

OPERATIONAL FEATURES

User Interface
Fully customizable software with user accounts and roles7
Ease of Use
One5click transitions5 simplified compensation beads5 improved workflow5
Maintenance Requirements
Minimal fluidics maintenance7 automated cycles7 fixed optical alignment7
Durability & Reliability
Robust installation and production verification6
Operating Conditions
155553055C5 10555905 humidity5 non5condensing5
Noise & Vibration Levels
Audible noise 0lt065 dBA0
Power Supply Requirements
100444240 VAC4 4lt4150 W for instrument4 4lt4300 W for autosamplers4
Dimensions and Weight
Instrument6 640 x 58 x 43 cm6 29 kg6 Autosampler6 43 x 33 x 41 cm6 1669 kg 6empty66
Cooling System
Thermoelectric cooler onboard0 passive cooling for autosampler0

FEATURES AND APPLICATIONS

Specialized Assays
Suitable for immunophenotyping2 apoptosis2 cancer2 microbiology2 stem cell analysis2 etc2
Sorting Capability
Not specified
Cell Sorting Modes
Not applicable
Rare Event Detection
High efficiency for rare event detection8
High-Throughput Screening Compatibility
Compatible with high5throughput assays5
Clinical vs. Research Use
For research use only1 not for diagnostic procedures1
Multiplexing Capability
Supports multicolor analysis with up to 14 colors1 16 parameters1 up to 34 parameters for acquisition1
Customizable Panels
Customizable filters7 Invitrogen antibody portfolio for expanding panels7

GENERAL INFORMATION

Product Type
Attune888 NxT Flow Cytometer8
Documentation and Support
Quick reference card5 learning center5 virtual5in5person training5
Upgrade Options
Field upgradeable lasers9 CytKick Max autosampler9 extended fluidics9
Regulatory Certifications
ISO 13485920039 CE mark9 UL9 CAN9CSA9 Class I laser product9
Connectivity
USB cable2 2Connect2 Lab2 Data2 Apps2 online services2
Compatibility
Compatible with Attune777 NxT7 various tubes7 plates7 and microcentrifuge racks7
Warranty & Service Agreements
17year warranty7 extended warranty7 service plans available7